Core F: Systems Biology & Biostatistics

核心 F：系统生物学

• 批准号: 10457727
• 负责人: Mark James Cameron
• 金额: $ 28.44万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10457727
• 关键词: Acquired Immunodeficiency Syndrome; Aftercare; Animal Model; Animals; Area; Basic Science; Bayesian Analysis; Big Data; Bioinformatics; Biological Assay; Biological Markers; Biometry; Biostatistics Core; Blood; Cells; Clinical; Clinical Research; Clinical Sciences; Clinical Trials; Collaborations; Collection; Complex; Computer Analysis; Consultations; Data; Databases; Development; Disease Outcome; Disease Progression; Experimental Designs; Fostering; Foundations; Gender; Gene Expression Profile; Genetic Transcription; Genomics; HIV; HIV Infections; Human; Image; Immune; Immune System Diseases; Immune response; Immunological Models; Immunologics; Immunology; Immunology procedure; Immunotherapeutic agent; Immunotherapy; Inflammation; Manuscripts; Maps; Metadata; Methodology; Mission; Molecular; Molecular Analysis; Outcome; Pathogenesis; Pathogenicity; Pathway interactions; Patients; Preparation; Prevention; Process; Protein Analysis; Protocols documentation; Publications; RNA analysis; Reporting; Reproducibility; Research; Research Design; Research Personnel; Sampling; Science; Services; Shapes; Statistical Data Interpretation; Structure; Support System; Surveys; Systems Biology; Technology; Tissue Model; Tissues; Training; Training and Education; Translational Research; Translations; Virus; Writing; advanced system; cohort; data acquisition; data dissemination; data management; data standards; deep sequencing; design; design verification; dysbiosis; fundamental research; high throughput analysis; immune function; immune reconstitution; innovation; interest; microbial; microbiome; microbiome research; microbiota; multidisciplinary; multiple omics; new technology; novel; public database; research study; sex; tool; transcriptome sequencing; transcriptomics; translational study; transmission process; treatment response

PROJECT SUMMARY/ABSTRACT –SYSTEMS BIOLOGY & BIOSTATISTICS CORE F The RUSTBELT CFAR Systems Biology & Biostatistics Core F provides support to a wide range of CFAR investigators by offering new multi-omic technologies, advanced biostatistics and computational analysis of “big data,” a stronger foundation in functional immunology studies, and a comprehensive ‘omic data management and training platform. The key mission of Core F is to provide systems biology approaches that allow for integration of different layers of host immune responses, from molecular triggers of immune responses to transcriptional signatures and interaction with resident microbiota. We will continue to support functional immunology studies that remain fundamental to HIV research. The Core assists investigators with research rigor and reproducibility through implementation of best practices for study design, analysis, and public sharing of results. In Aim 1 Core F will support genomics and microbiome research in HIV immunology and pathogenesis by providing users with a robust technological platform for transcriptional analysis by RNA-seq as well as deep sequencing in clinical and animal models of HIV infection. In Aim 2 Core F provides training and consultation in functional immunology as well as centralized access to state-of-the-art immunologic technologies, protocols, and training for the isolation, manipulation, culturing, and expansion of primary immune cells from blood and tissue, thereby supporting the RUSTBELT CFAR’s HIV transmission, gender research, cure, and pathogenesis translation objectives. In Aim 3 Core F facilitates all aspects of systems biology studies, by offering a centralized data acquisition, management, and reporting structure for all cohorts and ‘omics, using high-capacity assay and analysis workflows as well as publicly available ‘omic data to build publications and databases. Lastly, in Aim 4 This service function includes the collection and preparation of ‘omic and functional data for coordination and distribution to investigators, collecting associated de-identified patient annotations needed for analysis, and study publication. Core F Core F performs biostatistical correlate analysis to identify key drivers of protective immune responses, assist in designing validation strategies and writing manuscripts, and provide the metadata and data standardization pipelines for upload of results to public databases. By providing fundamental immune modeling support paired with multi-omic methodologies to identify key drivers or biomarkers of the host-virus interface in HIV, Core F will foster the complete systems biology approach necessary to support CFAR investigators in their translational research toward HIV cure, prevention, gender discrepancies, response to treatment, and the immunopathological landscape.

项目摘要/摘要-系统生物学与生物统计学核心