CORE B Symington

摘要 基因组不稳定核心的分子分析将为计划执行最先进的分析 研究人员分析细胞遗传学异常，以及DNA损伤反应和修复。这些 分析将补充测序结果(例如，检查未被DNA覆盖的重复区域 测序方法)，并为反复出现的基因组不稳定性和 使用基因组方法确定的重排(核心C)。此外，核心将执行基于单元的 检测和量化DNA损伤和DNA修复途径的生物标志物，并测量DNA的分析 修复中间体。为该计划配备专用核心是有好处的：1)成本效益；2) 根据每个项目的具体要求定制设计实验。通过将这些 在两位经验丰富的调查人员洛林·辛明顿博士和单查博士的领导下提供服务， CORE将通过向所有人提供基本服务来提高方案项目成员的生产力 项目，并确保数据采集和解释的一致性。核心有两个独立的具体 以核心领导人的专业知识为基础的目标。目标1的目的是开发和执行 染色体断裂、易位和拷贝数变化的细胞遗传学分析 标准G显带核型分析、光谱核型分析(SKY)、染色体涂色和位点特异性FISH。在……里面 第二个目标，核心将提供DNA损伤和反应因素的自动成像服务， 并将应用基于定量聚合酶链式反应的检测方法来检测由特定部位的末端切除形成的单链DNA 酵母和哺乳动物细胞中的DSB，以及DNA梳理法测量切割轨迹的长度 用辐射或拓扑异构酶抑制剂处理的细胞中的单分子。

SUMMARY The Molecular Analysis of Genomic Instability Core will perform state-of-the-art assays for program investigators in the analyses of cytogenetic aberrations, and DNA damage responses and repair. These analyses will complement sequencing results (e.g., examine repetitive regions that are not covered by DNA sequencing approaches), and provide experimental validation for recurrent genomic instability and rearrangements identified using genomic approaches (Core C). In addition, the core will perform cell-based assays to detect and quantify biomarkers for DNA damage and DNA repair pathways, and measure DNA repair intermediates. Having a dedicated core for this program is beneficial: 1) for cost effectiveness and 2) for custom designing the experiments based on specific requirement for each of the projects. By centralizing these services under the leadership of two experienced investigators, Drs. Lorraine Symington and Shan Zha, the Core will enhance the productivity of the program project members by providing essential services to all projects, and ensure uniformity of data acquisition and interpretation. The core has two independent specific aims, which are built upon the expertise of the core leaders. The purpose of Aim 1 is to develop and perform cytogenetic assays to characterize chromosomal breaks, translocations and copy number changes by standard G-band karyotyping, spectral karyotyping (SKY), chromosomal painting and locus-specific FISH. In the second aim, the Core will provide services for automated imaging of DNA damage and response factors, and will apply quantitative PCR-based assays for detection of ssDNA formed by end resection of site-specific DSBs in yeast and mammalian cells, and DNA combing method to measure the length of resection tracks on single molecules from cells treated with irradiation or topoisomerase inhibitors.