Clinical Science Research Core

项目摘要/摘要-临床科学研究核心 临床科学研究核心(CSRC)加强和促进前沿临床和翻译 通过两个不同但交互的子核心的操作进行营养/肥胖研究，这两个子核心提供一系列 通过具有凝聚力的一站式组织结构提供的服务和独特的培训 优质服务与培训融为一体。 临床研究绩效分核心通过以下方式协助研究人员：i)在设计 临床研究研究和解释核心分析产生的数据的结果，二)提供 生物统计学支持，以确定适当的样本量和适当的统计分析，三)测量 身体组成和能量消耗，四)制定和准备针对研究方案的膳食 用于饮食干预研究的代谢测试和外卖用餐，v)制备稳定的同位素标记 代谢示踪剂和激素注入以评估代谢功能，vi)提供高质量的临床 用于确定参与者资格、在干预期间监控参与者和评估的实验室测试 代谢功能，vii)获取肌肉和脂肪组织活检样本并插入桡动脉 用于采血的导管，viii)评估心血管功能，ix)进行锻炼和身体锻炼 性能测试，x)提供住院和门诊睡眠监测服务，xi)在以下方面提供协助 实施生活方式干预措施(饮食控制、减肥/增重、运动训练)；和 提供特定程序方面的培训，以协助研究人员进行临床研究。 代谢动力学分核心通过以下方式协助调查人员：i)在设计方案方面提供咨询，以 评估代谢动力学(例如，高胰岛素-正常血糖钳夹程序和频繁采样 静脉葡萄糖耐量试验结合稳定的同位素标记示踪剂输注进行评估 全身和组织细胞功能)以及对数据结果的解释 由岩心分析产生，二)生物样品的质谱分析，以确定示踪剂在 代谢底物和大分子，三)示踪剂和非示踪剂数据的数学模型以评估 代谢动力学，以及iv)提供特定分析方面的教育和培训。新陈代谢动力学分会- CORE提供这些服务的传统由来已久，过去一直专注于样本分析和 对特定代谢物进行建模以评估特定代谢途径(例如脂肪组织甘油三酯 脂解率、肝脏葡萄糖生成率、极低密度脂蛋白-甘油三酯和载脂蛋白B-100生成率)。这个 CORE现在扩展了这些服务，以包括快速发展的动态 代谢组学，这使得通过整个代谢网络评估代谢物流量成为可能。

PROJECT SUMMARY/ABSTRACT - CLINICAL SCIENCE RESEARCH CORE The Clinical Science Research Core (CSRC) enhances and facilitates leading-edge clinical and translational nutrition/obesity research through the operation of two distinct but interactive sub-cores that provide an array of services and unique training through a cohesive, “one-stop” organizational structure that ensures optimal integration of high-quality services and training. The Clinical Studies Performance sub-core assists investigators by: i) providing consultation in designing a clinical research study and in interpreting the results of the data generated by core analyses, ii) providing biostatistical support to determine adequate sample size and appropriate statistical analysis, iii) measuring body composition and energy expenditure, iv) developing and preparing study protocol-specific meals for metabolic testing and take-out meals for diet intervention studies, v) preparing stable isotopically labeled metabolic tracer and hormone infusates to assess metabolic function, vi) providing high-quality, clinical laboratory tests used to determine participant eligibility, monitor participants during interventions, and evaluate metabolic functions, vii) obtaining muscle and adipose tissue biopsy samples and inserting radial artery catheters for blood sampling, viii) evaluating cardiovascular function, ix) performing exercise and physical performance testing, x) providing inpatient and outpatient sleep monitoring services, xi) providing assistance in implementing lifestyle interventions (diet manipulation, weight loss/weight gain, exercise training); and xii) providing training in specific procedures to aid investigators in the performance of clinical studies. The Metabolic Kinetics sub-core assists investigators by: i) providing consultation in designing protocols to evaluate metabolic kinetics (e.g., the hyperinsulinemic-euglycemic clamp procedure and frequently sampled intravenous glucose tolerance test in conjunction with stable isotopically-labeled tracer infusions to evaluate whole-body and tissue- -cell function) and in interpreting the results of the data generated by core analyses, ii) mass-spectrometry analysis of biospecimens to determine tracer enrichment in metabolic substrates and macromolecules, iii) mathematical modeling of tracer and non-tracer data to assess metabolic kinetics, and iv) providing education and training in specific analyses. The Metabolic Kinetics sub- core has a long tradition of providing these services and in the past has focused its sample analysis and modeling on specific metabolites to evaluate specific metabolic pathways (e.g., adipose tissue triglyceride lipolytic rate, hepatic glucose production rate, VLDL-triglyceride and apolipoprotein B-100 production rate). The core has now expanded these services to include the new and rapidly evolving discipline of dynamic metabolomics, which makes it possible to evaluate metabolite flux through entire metabolic networks.