Tech Development Core

技术开发核心的目标是扩大高质量的原位杂交 (ISH)目前通过GEISHA可获得的表达数据集（鸡原位表达 杂交分析）数据库和用户界面，并将GEISHA数据集成到AviPort中。艺妓 是一个成熟的鸡胚社区资源，提供ISH基因表达 信息和相关基因组元数据，通过内部ISH筛选、文献策展和 从其他研究实验室获得表达数据。近年来，GEISHA项目 生成了50，000张完整的ISH图像和相关元数据的高质量数据集， 大约3,200个发育调控基因。随着许多生物医学相关的鸟类 研究发育的分子调控和直接实验分析，高质量ISH 这些资料对鸟类研究界特别有价值。虽然在过去的几年里， 一直是一个独立的资源，未来GEISHA将被集成到AviPort高吞吐量 ISH筛选将继续进行，因为该数据已被评为最高优先级， 高质量的基因组，为鸟类研究界。其他ISH信息将从 文献和其他研究人员获得。与Curation Core合作， 本体将被创建并用于注释ISH鸡胚图像。

The goal of the Technology Development Core is to expand the high quality in situ hybridization (ISH) expression dataset that is presently available through the GEISHA (gallus expression in situ hybridization analysis) database and user interface, and to integrate GEISHA data into AviPort. GEISHA is an established community resource for chicken embryo, providing ISH gene expression information and associated genomic metadata through in house ISH screens, literature curation and acquisition of expression data from other research laboratories. In recent years, the GEISHA project has generated a high quality dataset of 50,000 whole mount ISH images and associated metadata for approximately 3,200 developmentally regulated genes. With the focus of much biomedically related avian research on the molecular regulation of development and direct experimental analysis, high quality ISH information is of particularly high value to the avian research community. Though in past years GEISHA has been a stand-alone resource, going forward GEISHA will be integrated into AviPort High throughput ISH screens will continue to be conducted as this data has been voted the highest priority, after high quality genomes, for the avian research community. Additional ISH information will be curated from the literature and acquired from other researchers. In collaboration with the Curation Core, a chicken anatomical ontology will be created and used to annotate ISH chicken embryo images.