Imaging Core

项目摘要 耶鲁大学血液学卓越合作中心 成像核心 YCCEH的成像核心为良性血液学提供了最先进的显微镜技术 每年都有越来越多的用户。在此次续订中，我们建议通过增强功能继续运营 包括光毒性降低的长期时间流逝，适用于造血的超分辨率成像 组织和自动化高通量显微镜，用于在24小时内对1,000至10,000个细胞进行成像 决议。造血是一个复杂而动态的过程，涉及多种异种细胞类型。由于 成像数据中包含丰富的空间和/或时间信息，成像在以下方面具有独特的强大功能 揭示细胞动力学和组织结构对相关细胞和状态的影响。同样多的人 造血细胞体积小，粘附性小，很难从基因上进行操作，存在于深部 高度动态的和/或极其罕见的组织，能够将成像方法应用于造血 研究一直局限于拥有必要技术专长和仪器的少数几个实验室。 YCCEH影像芯旨在降低高技术门槛，推动影像应用 造血研究的技术。耶鲁大学是许多最先进的成像设备和 由世界知名的显微镜领导者和专家监督的设施。YCCEH成像核心将使 所有有研究兴趣的研究人员都可以很容易地接触到大量的成像平台 地方性或全国性的造血。YCCEH影像核心将继续为其成员提供访问 最先进的仪器，包括广域成像、共焦成像、双光子成像和流式细胞术成像。重要的是 通过这一成像核心，我们正在将其作为一项服务提供，并在使用创新技术方面提供访问和专业知识 成像方式包括自动长期活细胞延时显微镜，组织的光学清除和 超分辨率显微镜。CORE的董事、比尔斯多夫博士和郭博士将担任 YCCEH成员和参与的设施和实验室，并将帮助确定最适合的技术 个人研究项目。成像核心还将支持两名兼职成像专家，他们将接受培训 YCCEH成员，维护比尔斯多夫和郭实验室提供的仪器并协助 试剂、样品制备和数据分析。我们将继续推动知识的传播 通过组织研讨会、讲习班、研讨会和培训，在造血研究中进行成像 侧重于学生、实习生和其他早期调查人员。此外，成像核心将 继续开发、优化和传播适合分析的创新型记者/探头 通过荧光成像和/或流式细胞术检测造血细胞。我们会继续鼓励基督教青年会 会员通过用户激励对其研究项目的各种显微技术进行评估 计划，通过消除将资金投资于一个陌生的技术领域的心理障碍。 总体而言，成像核心将促进并使单个造血研究人员能够进行观察 利用能够提供独特的结构、功能的方法 和/或动态信息。

Project Summary Yale Cooperative Center of Excellence in Hematology Imaging Core The Imaging Core of the YCCEH has provided access to state-of-the-art microscopy for benign hematology to progressively more users each year. In this renewal, we propose to continue operations with enhancements including longterm timelapse with decreased phototoxicity, adapted super resolution imaging for hematopoietic tissues and automated high-throughput microscopy for imaging of 1,000 to 10,000 cells/24 hrs at super- resolution. Hematopoiesis is a complex and dynamic process involving many heterologous cell types. Due to the rich spatial and/or temporal information contained in imaging data, imaging is uniquely powerful in revealing the influences of cellular dynamics and tissue organization on the relevant cells and states. As many hematopoietic cells are small in size, minimally adhesive, difficult to manipulate genetically, reside in deep tissues, highly dynamic and/or exceedingly rare, the ability to apply imaging approaches to hematopoiesis research has been limited to few laboratories with the requisite technical expertise and instrumentation. YCCEH Imaging Core aims to reduce the high technical hurdle and promote the application of imaging techniques for hematopoietic research. Yale is the home of many state-of-the-art imaging equipment and facilities overseen by world-renowned leaders and experts in microscopy. The YCCEH Imaging Core will make the large array of imaging platforms readily accessible to all investigators with a research interest in hematopoiesis, locally or nationally. YCCEH Imaging Core will continue to provide its members with access to state-of-the-art instrumentation including widefield, confocal, 2-photon and flow cytometry imaging. Importantly, through this Imaging Core, we are making it available as a service, access to and expertise in using innovative imaging modalities including automated long-term live-cell time-lapse microscopy, optical clearing of tissue and super-resolution microscopy. The directors of the Core, Drs. Bewersdorf and Guo will act as liaisons between YCCEH members and the participating facilities and labs and will help identifying most suitable techniques for individual research projects. The Imaging Core will also support two part time imaging specialists who will train YCCEH members, maintain the instruments provided by the Bewersdorf and Guo labs and assist with reagents, sample preparation and data analysis. We will continue to promote the dissemination of knowledge for imaging in hematopoietic research by organizing symposia, workshops, seminars and training with an emphasis on students, trainees and other early-stage investigators. Furthermore, the Imaging Core will continue to develop, optimize and disseminate innovative reporters/probes that are suitable for analyzing hematopoietic cells by fluorescence imaging and/or flow cytometry. We will continue to encourage YCCEH members to evaluate various microscopy techniques for their research projects through the user incentive program, by eliminating the psychological hurdle of investing funds into an unfamiliar technical domain. Overall, the Imaging Core will facilitate and enable individual hematopoietic researchers to make observations of their specific hematopoietic cell linages, using approaches that can provide unique structural, functional and/or dynamic information.