Mechanisms of Basal Forebrain Control Over Sensory Processing

项目总结 家长奖的首要目标是发展对神经回路如何在 基底前脑影响感觉加工。对此，建议的目的是在家长奖励测试中 关于注意、唤醒和奖励预测对嗅觉加工影响的假说 基底前脑内定时的胆碱能信号控制向嗅球的投射输出。这 这一假说正在使用病毒基因工程、转基因小鼠模型和体内实验相结合的方法进行验证。 双光子成像、纤维光度学、老鼠行为和切片电生理学。这些复合体 实验范式在产生小鼠、进行手术、恢复、 培训和数据收集。暂停产假试验不仅会扰乱正在进行的 实验，但这将严重影响未来需要育种、准备和培训的实验 老鼠的世界。这项补充建议旨在避免在产假期间严重中断连续性。 通过提供(1)一名为期九个月的兼职技术员和(2)数据存储服务器 允许跨多个用户进行安全、远程访问和协作的硬件。技术人员的角色将 首先是保持正在进行的小鼠实验，因为PI完全处于休假状态。然后，当PI休假归来时 兼职时，技术人员的角色将转变为维护实验、处理数据和 为未来的切片电生理学实验准备小鼠。最后，当PI返回全职工作时，角色 的技术人员将保持行为实验的连续性，在这种情况下 由于儿童保育的不可预见性，在PI方面需要安排时间。同时， 实施可远程访问的数据存储服务器将使 技术员和PI休假期间和复工后全职。这些资源加在一起将 帮助最大限度地提高生产率，并在父母产假期间促进父母奖励的连续性 奖励圆周率。

PROJECT SUMMARY The overarching goals of the parent award are to develop a mechanistic understanding of how neural circuits in the basal forebrain impact sensory processing. Toward this, the proposed Aims in the parent award test the hypothesis that effects of attention, arousal, and reward prediction on olfactory processing rely on precisely timed, cholinergic signaling within the basal forebrain controlling projection output to the olfactory bulb. This hypothesis is being tested using a combination of viral genetic engineering, transgenic mouse models, in vivo two-photon imaging, fiber photometry, mouse behavior, and slice electrophysiology. These complex experimental paradigms have relatively long timelines for generating mice, performing surgeries, recovery, training, and data collection. Pausing experimentation for maternity leave would not only disrupt ongoing experiments, but it would seriously impact future experiments requiring the breeding, preparation, and training of mice. This supplement proposal seeks to avoid a significant disruption in continuity during the maternity leave of the parent award PI by providing for (1) a part-time technician for nine months and (2) data storage server hardware allowing secure, remote access and collaboration across multiple users. The role of the technician will first be to maintain ongoing mouse experimentation as the PI is fully on leave. Then, as the PI returns from leave part time, the role of the technician will shift to a combination of maintaining experiments, processing data, and preparing mice for future slice electrophysiology experiments. Finally, as the PI returns to work full time the role of the technician will be to maintain the continuity of behavioral experimentation in the case that flexible scheduling is required on the part of the PI due to the unpredictable nature of childcare. At the same time, implementing a remotely accessible data storage server will enable real time collaboration between the technician and the PI for the duration of leave and after returning to work full time. Together these resources will help maximize productivity and promote continuity of the parent award during the maternity leave of the parent award PI.