Metabolic basis of mosquito-endosymbiont-virus interactions RBL Admin Supplement

项目摘要/摘要 本补编是对特别关注通知的回应，不是-AI-22-049， 对于NIAID区域生物遏制实验室(RBL)和其他在RBL进行研究的NIH受赠者- 附属网站“和我们的NIAID赠款的范围(基金R01AI151166)，题为”蚊子的代谢基础-- 内共生体与病毒的相互作用。在这里，我们将加速和推进家长奖的具体目标1-3 通过两个主要的补充目标；SA1-3Supp1：评估细胞脂质的修改如何改变 Ae病毒包膜的组成和成熟和感染性所需的结构转变。埃及伊蚊。 从SA 1-3生成的数据将显著增强父级奖励数据结果，因为它提供 对脂质调节如何改变病毒结构和功能从而影响病毒寿命的生物物理洞察 在Ae中循环。埃及伊蚊。SA 2supp2：评估沃尔巴克氏菌介导的血脂变化如何改变ZIKV和CHIKV感染 和病毒相关的脂质调节。从SA 2生成的数据将显著增强父代 通过提供对感染后载体的代谢状态的更多洞察来奖励数据结果 这三种载体病毒都是。这些研究将增强我们对代谢瓶颈的理解，这些瓶颈可能是 适用于所有三种病毒，和/或可能阻碍生物防治工作的重大差异。这两种努力 与RBL在(诊断、治疗、疫苗和传播控制方法)方面的目标协同 针对由Ae.埃及)；并得到RMRBL科学主任的全力支持。

PROJECT SUMMARY / ABSTRACT This supplement is responsive to the Notice of Special Interest, NOT-AI-22-049, “Administrative Supplements for NIAID Regional Biocontainment Laboratories (RBL) and other NIH Grantees Conducting Research at RBL- affiliated Sites” and the scope of our NIAID grant, (fund R01AI151166), entitled, “Metabolic basis of mosquito- endosymbiont-virus interactions.” Here, we will accelerate and advance Specific Aims 1-3 of the parent award through two primary supplementary aims; SA 1-3.supp1: Assess how modification of cellular lipids alters the composition of the viral envelope and required structural transitions for maturation and infectivity in Ae. Aegypti. The data generated from SA 1-3.supp1 will significantly enhance the parent award data outcomes as it provides biophysical insight into how lipid modulation might alter virus structure and function and thus impact the viral life cycle in Ae. aegypti. SA 2.supp2: Assess how Wolbachia-mediated lipid changes alter ZIKV and CHIKV infection and virus-associated lipid modulations. The data generated from SA 2.supp2 will significantly enhance the parent award data outcomes by providing additional insight into the metabolic state of the vector following infection with all three vectored viruses. The studies will enhance our understanding of metabolic choke points that may be applicable to all three viruses, and/or significant differences that might hinder biocontrol efforts. Both efforts synergize with the goals of the RBL in (diagnostics, therapeutics, vaccines, and transmission control methods for Arboviruses vectored by Ae. aegypti); and has the full support of the Scientific Director of the RMRBL.