Structural and Drug Screening (SDS)

项目摘要/摘要(结构和药物筛选共享资源) 结构和药物筛选共享资源(SDS-SR)提供围绕关键字的服务 通过先导优化的早期药物开发要素。SDS-SR是结构化的 生物学(SB)、高通量筛选(HTS)和药物化学(MC)。SB、HTS和MC频繁 协调罗杰尔癌症中心(Rogel)成员的服务，以提高癌症的效率和影响- 相关项目。SDS-SR使Rogel成员能够使用先进的基于结构的药物设计和/或 无偏见的HTS策略，可通过里程碑驱动的研究来识别可以推进的化学物质 导致高效和有效地发现和开发精确肿瘤学药物的计划-战略 罗杰尔癌症中心的目标。SDS-SR的总体目标是识别新的分子靶点 用于癌症治疗，并确定与这些靶点相互作用的新的化学先导和/或工具分子。 我们的服务始于通过高通量克隆和确认表达来提炼蛋白质靶标 并通过X射线结晶学对HIT结合进行优化。一旦目标被提纯，它就会被大量提纯 范围从微克到克数，用于高通量筛选(HTS)分析开发和大规模HTS 进行命中鉴定。提供了额外的生物物理正交分析来确定该化合物是否 在结构确定之前直接与靶结合。罗杰尔成员接受了培训， 在流程的每一步都要提供建议、咨询和专门的设备。SDS-SR服务包括 由在学术界和工业界拥有广泛专业知识和背景的科学工作人员提供。许多. 这些服务不能通过供应商或其他机构提供。SDS-SR仍然是一个无价的 为罗杰尔成员提供资源。SDS-SR具有凝聚力的活动、服务和流程确保项目 从基础科学发现到早期抗癌药物发现，都是沿着转换管道向前推进的。

PROJECT SUMMARY/ABSTRACT (STRUCTURE AND DRUG SCREENING SHARED RESOURCE) The Structure and Drug Screening Shared Resource (SDS-SR) provides services encompassing the key elements of early-stage drug development through lead optimization. SDS-SR is a collaboration of Structural Biology (SB), High-Throughput Screening (HTS), and Medicinal Chemistry (MC). SB, HTS, and MC frequently coordinate services for Rogel Cancer Center (Rogel) members to increase the efficiency and impact of cancer- related projects. SDS-SR enables Rogel members to use advanced structure-based drug design and/or nonbiased HTS strategies to identify chemical matter that can be advanced through a milestone-driven research plan resulting in efficient and effective discovery and development of precision oncology medicines - a strategic goal of the Rogel Cancer Center. The overall goals of SDS-SR are to identify novel molecular targets for new cancer therapeutics and to identify novel chemical leads and/or tool molecules that interact with these targets. Our services start with refining the protein target via high throughput cloning and expression through confirmation and optimization of hit binding via X-ray crystallography. Once the target is refined, it is purified in quantities ranging from micrograms to grams for high-throughput screening (HTS) assay development and large scale HTS for hit identification. Additional biophysical orthogonal assays are offered to determine whether the compound binds directly to the target prior to structure determination. Rogel members are provided with training, advisement, consultation, and specialized equipment through every step of the process. SDS-SR services are provided by scientific staff with a breadth of expertise and backgrounds in both academia and industry. Many of these services are not available through vendors or other institutions. SDS-SR continues to be an invaluable resource to Rogel members. The SDS-SR’s cohesive activities, services, and processes ensure that projects are advanced along the translational pipeline, from basic science discoveries into early cancer drug discovery.