Biomedical Resource Core

摘要 约翰霍普金斯大学奥布莱恩中心促进肾脏健康公平的生物医学资源核心(BRC) 将提供一系列创新的研究服务、资源和工具，供调查人员了解 并改善肾脏疾病方面的差异，最终实现肾脏健康公平。重点放在 饮食和社会压力是导致肾脏健康差异的原因，BRC是专门为解决这一问题而设计的 具有广泛影响的根本性挑战，推动全国肾脏研究。临床科学饮食 和社会应激源实验室(C-DSSL)，由劳伦斯·阿佩尔博士领导，以及基础科学饮食和 由保罗·韦林博士领导的社会应激源实验室(B-DSSL)提供专家咨询，经过验证 礼宾、培训、获取材料和设备；并将进行试点研究或更大规模的研究 项目。联合咨询服务和深思熟虑的团队方法将两个实验室结合在一起，并创建了一个 创新的双向管道，最大限度地提高翻译效率。C-DSSL将提供以下服务：1) 临床研究设计；2)饮食评估；3)获得大量大型队列研究，包括种族和 社会经济多样化的人群，深入的表型和饮食数据的收集，储存的生物样本， 和较长的随访期；4)以停止高血压的饮食方法试验为模型的喂养研究； 5)行为干预研究；6)社区中的翻译研究；7)使用公共可用的 数据集；以及 8)生物统计学支持。B-DSSL将为调查人员提供全面的服务和 用于临床前研究的资源，以确定人类健康差距的机制基础 应激源，以小鼠为模型系统；服务将包括：1)研究设计；2)人性化小鼠饮食 3)一个完整的小鼠肾脏表型核心，从整体上探索应激机制 动物生理学到分子机制；4)脂肪和碳水化合物代谢表型核心， 配备最先进的代谢量热笼；5)研究免疫学的核心 饮食和社会应激源在肾脏中的表现；6)具有最先进细菌的微生物核心- 免费设施；以及7)饮食和社会压力模型的小鼠生物库。所有共享服务和资源 将接受严格的质量控制、验证以及对需求和影响的定期审查。除 通过实验室提供的服务及其综合方法，BRC将促进培训和能力 建立最大限度地让来自不同领域的早期调查人员进入肾脏健康公平性研究。 BRC致力于与该财团广泛合作，并自由分享其专业知识 资源。

ABSTRACT The Biomedical Resource Core (BRC) of the Johns Hopkins O’Brien Center to Advance Kidney Health Equity will provide an innovative portfolio of research services, resources, and tools for investigators to understand and ameliorate disparities in kidney disease, towards ultimately achieving kidney health equity. With a focus on dietary and social stresses that drive kidney health disparities, the BRC is uniquely designed to address this fundamental challenge with a broad impact, advancing kidney research nationally. A Clinical Science Dietary and Social Stressor Laboratory (C-DSSL) directed by Dr. Lawrence Appel, and a Basic Science Dietary and Social Stressor Laboratory (B-DSSL), directed by Dr. Paul Welling provide expert consultation, proven protocols, training, access to materials and equipment; and will execute pilot studies or larger research projects. A joint consultation service, and a deliberate team approach meld the two laboratories, and create an innovative bidirectional pipeline that maximizes translation. The C-DSSL will provide the following services : 1) clinical study design; 2) dietary assessment; 3) access to numerous large cohort studies, with racially and socioeconomically diverse populations, deep phenotyping and collection of dietary data, stored biospecimens, and long follow-up periods; 4) feeding studies modeled on the Dietary Approaches to Stop Hypertension trials; 5) behavioral intervention studies; 6) translational studies in the community; 7) use of publicly available datasets; and 8) biostatistical support. The B-DSSL will provide investigators with comprehensive services and resources for preclinical research to determine the mechanistic underpinnings of human health disparity stressors, using mice as a model system; services will include: 1) study design; 2) humanized mouse diet protocols; 3) a comprehensive mouse kidney phenotyping core for exploring stress mechanisms from whole animal physiology to molecular mechanism; 4) a lipid and carbohydrate metabolism phenotyping core, equipped with state-of-the-art metabolic cages for calorimetry; 5) a core for studying immunological manifestations of dietary and social stressors in the kidney; 6) a microbiome core with a state-of-the-art germ- free facility; and 7) a mouse biobank of dietary and social stress models. All shared services and resources will undergo strict quality control, validation, and periodic review of demand and impact. In addition to the services provided by the laboratories and their integrated approach, the BRC will foster training and capacity building to maximize entry of early-stage investigators from diverse fields new to kidney health equity research. The BRC is committed to collaborating with the consortium broadly, and to freely sharing its specialized resources.