Mouse Histology and Phenotyping Laboratory Shared Resource

摘要-小鼠组织学和表型实验室 小鼠组织学和表型实验室(MHPL)成立于2009年，最初是一个发展中的 Lurie癌症中心(LCC)的核心实验室，并在最后一次竞争后成为公认的核心 2013年续签CCSG。核心提供LCC成员和西北大学(NU)的研究 由训练有素的病理学家对实验研究动物组织进行组织病理学评估的社区 专业的组织技术专家。MHPL的使命是为研究人员提供高度专业化的基于组织的 组织学和表型鉴定服务，以支持他们基于动物的研究工作。自上次参赛以来 更新后，该实验室扩大了其服务范围，现在为 研究动物种类繁多。表型鉴定服务包括免疫组织化学(IHC)， 免疫荧光(IF)，完整的小鼠尸检，器官和组织的详细表型，以及组织- 基于毒物学分析。该实验室还为学生、博士后研究员、 工作人员和研究人员学习组织学和表型分析技术。MHPL董事是一个董事会- 注册病理学家，并可协助有限责任公司调查人员制定最佳策略以阐明 特定的表型，获得关于目标分子生物行为的机械性见解，并进行研究 外源性给药物质对啮齿动物的毒性和/或治疗效果。 MHPL提供的全面组织病理学支持服务增强了LCC的能力 研究人员将鉴定存活的和胚胎致死的小鼠模型，并开发和分析新的体内实验 癌症生物学、预防和治疗研究的模型系统。LCC成员对MHPL的使用情况 在本供资期间大幅增加。在过去的一年里，核心提供了服务 74个LCC成员，其中69个由同行审查资助，其工作产生了多重高度影响 出版物。

ABSTRACT – MOUSE HISTOLOGY AND PHENOTYPING LABORATORY The Mouse Histology and Phenotyping Laboratory (MHPL) was originally established in 2009 as a developing core laboratory for the Lurie Cancer Center (LCC), and emerged as an established core after the last competing CCSG renewal in 2013. The core provides LCC members and the Northwestern University (NU) research community with histopathologic assessment of experimental research animal tissues by trained pathologists and expert histotechnologists. The mission of MHPL is to provide investigators with highly specialized tissue-based histology and phenotyping services to support their animal-based research work. Since the last competing renewal, the laboratory has expanded its services and now provides comprehensive histology services for a wide variety of research animal species. Phenotyping services include immunohistochemistry (IHC), immunofluorescence (IF), whole mouse necropsy with detailed phenotyping of organs and tissues, and tissue- based toxicology analyses. The laboratory also provides training opportunities for students, post-doctoral fellows, staff and research faculty to learn histology and phenotyping analysis techniques. The MHPL Director is a board- certified pathologist and is available to assist LLC investigators in developing the best strategies to elucidate specific phenotypes, gain mechanistic insights regarding the biologic actions of targeted molecules, and study the toxicity and/or therapeutic efficacy of exogenously administered substances in rodents. The comprehensive histopathology support services provided by the MHPL enhance the ability of LCC investigators to characterize viable and embryonic lethal mouse models, and to develop and analyze new in vivo model systems for studies of cancer biology, prevention, and therapy. The use of the MHPL by LCC members has increased substantially over the current funding period. During the last year, the core provided services to 74 LCC members, of which 69 were peer review funded and whose work resulted in multiple high impact publications.