NHGRI/DIR Microarray and single-cell Core

微阵列和单细胞基因组学核心： NHGRI微阵列和单细胞基因组核心设备代表NHGRI、NIMH和NINDS之间的联盟。它为内部调查人员提供全面的服务，具有成本效益和时间效率，获得最先进的基因组学和转录组学技术，以了解基因组拷贝数和基因表达模式。大多数研究人员的项目请求都是Full Service，其中研究人员将提供芯片、标记试剂盒和分离的RNA样本，核心将进行标记、杂交和数据提取。项目完成后，向调查员提供一份总结报告，其中包括数据质量评估、初步分析和原始数据输出文件。该核心还应要求为调查人员提供任何平台和专门的核糖核酸协议提取、数据质量控制、数据分析、数据挖掘和数据故障排除方面的实践培训。对于单细胞测序和RNA测序，用户将负责提供测序试剂盒，核心将提供用于制作DNA文库的试剂。对于单细胞RNA-Seq，用户将负责提供一些液滴生成试剂和测序试剂盒。在RNA或细胞悬液通过QC后，将生成DNA文库，然后在测序之前进行另一次QC。核心将提供测序QC和FASTQ原始测序文件。PI将提供一个基本空间链接，以查看和下载所有原始和分析文件。对于单一单元，将向请求者提供数据质量控制和初步分析，以及关于Partek-Flow软件使用的教程。

Microarrays and Single-Cell Genomics Core: The NHGRI Microarrays and Single-Cell Genomics Core Facility represents a consortium between NHGRI, NIMH, and NINDS. It provides intramural investigators with full service, cost-effective and time-efficient access to state-of-the-art genomics and transcriptomics technologies for understanding genome copy number and patterns of gene expression. Most investigator project requests are Full Service, in which the investigator will provide the chips, labeling kit and isolated RNA samples, and the core will perform labeling, hybridization and data extraction. Upon project completion, the investigator is provided with a summary report including the data quality assessment, preliminary analysis and raw data output files. The core also provides hands-on training to investigators, upon request, for any of the platforms and specialized RNA protocol extraction, data quality control, data analysis, data mining and data troubleshooting. For single-cell sequencing and RNA-sequencing, users will be responsible for providing the sequencing kit and the core will provide the reagents for making the DNA libraries. For single-cell RNA-Seq, the users will be responsible for providing some reagents for droplet generation and the sequencing kit. After the RNA or cell suspension passes QC, DNA libraries will be generated and then subject to another QC before sequencing. The core will provide the sequencing QC and the FASTQ raw sequencing files. The PI will be provided with a BaseSpace link to view and download all raw and analysis files. For single-cell, the requester will be provided with the data QC and preliminary analysis and a tutorial on Partek-Flow software use.