Sample Processing and Analysis

摘要 该核心的任务是促进由所有计划执行的翻译和基于实验室的研究 调查人员。该核心支持4个功能：1)原始AML和MDS的处理和冷冻保存 样本；2)用病理、细胞遗传学和流式细胞术数据对原始样本进行注释；3)注释 具有基因组和功能数据的原代AML样本；以及4)维护AML细胞系、原始移植物的库 适合于功能分析的体外模型。截至2018年11月，已有12790个骨髓样本 来自3,590名独特的血液系统恶性肿瘤患者。所有这些样本都是从 来自参加IRB批准的样本采集方案DF/HCC01-206的患者：血液和骨髓 恶性血液病、骨髓疾病患者的研究样本收集 和正常的捐赠者。该银行包括1473名AML患者和634名MDS患者的样本。这个 在过去的五年中，样本被广泛用于支持临床和实验室研究 药物开发、癌基因功能和预后分析、BH3图谱的开发、创建 大量基因定义的急性髓系白血病PDX模型和其他实验室研究。

ABSTRACT This core's mission is to facilitate translational and laboratory-based research performed by all program investigators. The core supports 4 functions: 1) processing and cryopreservation of primary AML and MDS samples; 2) annotation of primary samples with pathologic, cytogenetic and flow cytometry data; 3) annotation of primary AML samples with genomic and functional data; and 4) maintain a bank of AML cell lines, primagrafts and in vitro models suitable for functional assays. As of November 2018, 12,790 bone marrow samples have been banked from 3,590 unique patients with hematologic malignancies. All of these samples were obtained from patients enrolled on IRB-approved sample collection protocol DF/HCC 01-206: Blood and Bone Marrow Sample Collection for Research Studies in Patients with Hematologic Malignancies, Bone Marrow Disorders, and Normal Donors. The bank includes samples from 1473 patients with AML and 634 patients with MDS. The samples have been extensively used to support clinical and laboratory research over the last five years in support of drug development, analysis of oncogene function and prognosis, development of BH3 profiling, creation of a large bank of genomically defined PDX models of AML, and other laboratory studies.