PROTEINS OF NORMAL AND CATARACTOUS LENSES

描述（改编自申请人摘要）：总体目标 的应用是氧化应激的作用，在评估 人类老年性白内障的发生。 该应用程序将研究 核性白内障豚鼠模型中核性白内障的形成 在人类衰老过程中形成。 豚鼠形成核性白内障， 对高压氧的反应 传统技术如拉曼 光谱和二维凝胶电泳，SDS PAGE和 免疫印迹分析将与更创新的方法相结合， 作为由M. 卢，一个合作者。 这些分析将提供关于 膜相关聚集和可溶性的相对贡献 核性白内障晶状体蛋白聚集致混浊 高压氧/豚鼠模型。 虽然应用程序的元素 例如基因敲除和转基因动物的研究， 不成熟，应用一般是非常强大和重要的新 可以预期关于白内障形成的信息。

DESCRIPTION (Adapted from applicant's abstract): The overall objective of the application is the evaluation of the role of oxidative stress in the development of human senile cataract. The application will study formation of nuclear cataract in a guinea pig model for nuclear cataract formation in aging humans. The guinea pig forms a nuclear cataract in response to hyperbaric oxygen. Traditional techniques such as Raman spectroscopy and 2-dimensional gel electrophoresis, SDS PAGE and immunoblot analyses will be combined with more innovative methods such as LC assays for disulfide bonds pioneered for lens proteins by Dr. M. Lou, a collaborator. The analyses will provide information on the relative contribution of membrane-associated aggregation and soluble crystallin aggregation to opacification in nuclear cataract of the hyperbaric oxygen/guinea pig model. While elements of the application such as the studies with knockout and transgenic animals may be premature, the application is generally very strong and important new information on cataract formation can be expected.