REGULATION OF THE OVINE FSH BETA GENE BY ESTROGEN

描述（改编自研究者摘要）：基于 激活的E受体（ER）通过与一个 或多种直接结合-105/-72 DNA的转录因子， 提出了以下目标：目标1寻求定义DNA序列 E抑制FSH β所必需的; Aim 2将识别细胞核中的 直接结合到-105到-72 bp区域的蛋白质， 它们与激活的ER相互作用; Aim 3将使用现有的突变体， ER定义镇压所需的特征;目标4 包括产生转基因小鼠，所述转基因小鼠含有野生型或 突变的绵羊FSH β基因，以确定哪些结合位点， -105/-72序列是体内负调控的关键。

DESCRIPTION (Adapted from the Investigator's Abstract): Based on the hypothesis that activated E receptor (ER) functions via association with one or more transcription factors that bind directly to -105/-72 DNA, the following aims are proposed: Aim 1 seeks to define the DNA sequences necessary for repression of FSH beta by E; Aim 2 will identify the nuclear proteins that bind directly to the -105 to -72 bp region and determine how they interact with the activated ER; Aim 3 will use existing mutants of the ER to define the characteristics necessary for repression; and Aim 4 involves the production of transgenic mice that contain either wild-type or mutated ovine FSH beta genes to determine which binding sites in the -105/-72 sequence are critical for negative regulation in vivo.