A broad catalog of transcriptomic and proteomic markers for human cell senescence

衰老细胞的积累是衰老衰退和衰老相关疾病的驱动力。尽管细胞衰老在培养细胞中已经有了详细的定义，但目前对体内衰老的了解非常有限，部分原因是组织和器官中细胞的生物体的复杂性。为了更好地了解生物体内的衰老，我们建议在人类原代细胞的一个大的子集中创建一个全面的衰老模型目录，在其中我们将描述转录组、蛋白质组和分泌组。这一特征将使共享和器官/谱系特异性衰老标志物的识别成为可能，从而发现用于研究体内细胞衰老的标志物。最后，我们计划在众所周知的体内衰老模型中验证所有这些发现，例如衰老和化疗诱导的衰老。我们预计，该项目的完成将为更系统地研究细胞衰老及其与衰老生理和疾病的关系铺平道路。

The accumulation of senescent cells is a driving force of aging decline and age-related diseases. Even though cellular senescence has been defined in detail in cultured cells, current knowledge of senescence in vivo is very limited, due in part to the complexity of organisms of cells in tissues and organs. To gain a better understanding of senescence in vivo in the organism, we propose to create a comprehensive catalog of senescence models in a large subset of human primary cells, where we will profile the transcriptome, proteome, and secretome. This characterization will enable the identification of both shared and organ/lineage-specific senescence markers, to uncover markers to study cellular senescence in vivo. Finally, we plan to validate all these findings in well-known in vivo models of senescence, such as aging and chemotherapy-induced senescence. We anticipate that the completion of this project will pave the way for a more systematic study of cellular senescence and its connections to aging physiology and disease.