Mouse Cardiac Physiology and Surgical Core (Core C)

摘要 小鼠是一种强大的模式生物，可以解剖潜在的分子和 导致心力衰竭或缺血性损伤的细胞丢失的遗传机制。这个 老鼠将是所有四个项目中提出的所有基因与疾病关系研究的基础 在拟议的计划中。Core C将系统地制作心脏损伤模型 确保所有4个项目的可重复性和可比性，从而使结果 不受模型变异性的影响。鼠标将根据需要在达拉斯和达拉斯之间运送 针对辛辛那提提出的模型进行制作和分析。心脏病的表型 还将在核心C中进行广泛的分析，如结构和功能评估， 这也将以受控和可重现的方式在4个 项目，核心C将提供缺血再灌注损伤(I/R)的小鼠模型， 横主动脉致心肌梗死损伤(MI)、心脏压力超负荷 收缩(TAC)和心尖切除早期新生小鼠心脏。对于表型 分析这些小鼠模型，核心C将进行超声心动图，有创性 使用压力感应脑室导管的血流动力学和心脏磁学 磁共振成像(MRI)。所有4个项目都将广泛使用Core C，这两个外科手术 模型生成能力和功能评估能力。技术高超 核心人员将确保手术一致性以及数据集成、一致性和 4个项目的透明度。核心C方法和服务对于 心血管结构和功能的分析将提供不可或缺的 基础和公共链接点

Abstract The mouse is a powerful model organism for dissecting the underlying molecular and genetic mechanisms leading to heart failure or cellular loss with ischemic injury. The mouse will be the basis for all gene-disease relationship studies proposed in all 4 Projects in the proposed Program. Core C will produce models of cardiac injury in a systematic manner to ensure reproducibility and comparability across all 4 Projects so that results are not influenced by model variability. Mice will be shipped as needed between Dallas and Cincinnati for the proposed model production and analyses. The heart disease phenotype will also be extensively analyzed in Core C, such as structure and functional assessment, which will also be performed in a controlled and reproducible manner between the 4 projects, Core C will provide mouse models of ischemia-reperfusion injury (I/R), myocardial infarction injury (MI), cardiac pressure overload induced by transverse aortic constriction (TAC), and apical resection of the early neonatal mouse heart. For phenotypic analysis of these mouse models, Core C will perform echocardiography, invasive hemodynamics using a pressure sensing intraventricular catheter, and cardiac magnetic resonance imaging (MRI). All 4 Projects will extensively use Core C, both the surgical model generation capabilities and the functional assessment capabilities. Highly skilled core personnel will ensure surgical consistency as well as data integration, uniformity, and transparency across the 4 Projects. Core C approaches and services are essential for cardiovascular structure and function analyses and will provide an indispensable foundation and common linking point