CYTOKINE REGULATION OF EARLY EVENTS IN BLOOD VESSEL FORM

描述(改编自《调查者摘要》)：一个基本过程 在血管的形成(血管生成)是网络的融合 内皮管形成更大的血管。这个过程从头到尾都在发生 胚胎发育，也许最好的例证是 单个背主动脉来自内皮管网络。 沿着胚胎轴。这种融合过程背后的机制是 未知。外源导入血管内皮细胞生长因子 血管内皮生长因子(VEGF)可以促进网络的超融合。超融合是一个过程 哪些微血管过度融合。此外，还有一项研究发现，血管内皮生长因子 它的受体存在于胚胎的血管生成区域 血管内皮细胞生长因子的生物利用度受到严格的调控 发展。实验旨在证实血管内皮细胞生长因子作为 血管融合的体内调节剂。建议的一个主要特点是 实验将在活体内进行，在井的背景下 以血管化区域为特征。血管内皮细胞生长因子的作用将通过 血管内皮生长因子受体拮抗剂的一系列实验 相互作用将被用来扰乱血管生成的正常模式。 这项工作的结果可能有助于更好地理解 血管形成不仅发生在胚胎发育中，也发生在 伤口愈合，以及与血管生成相关的疾病，如眼睛 新生血管、肿瘤生长和血管瘤。

DESCRIPTION (Adapted from Investigator's Abstract): A fundamental process in the formation of blood vessels (vasculogenesis)is the fusion of networks of endothelial tubes to form larger vessels. This process occurs throughout embryonic development and is perhaps best illustrated in the formation of the individual dorsal aortae from networks of endothelial tubes present along the embryonic axis. The mechanism underlying this fusion process is unknown. Exogenously introduced vascular endothelial cell growth factor (VEGF) can promote hyperfusion of networks. Hyperfusion is a process in which microvessels fuse excessively. This along with the finding that VEGF and its receptors are present in vasculogenic regions of the embryo suggest that the bioavailability of VEGF is strictly regulated during vascular development. Experiments are designed to substantiate the role of VEGF as an in vivo regulator of vascular fusion. A major feature of the proposed experiments is that they will be done in vivo, in the context of well characterized vascularizing regions. The role of VEGF will be evaluated by a series of experiments in which antagonists of the VEGF receptor interactions will be used to perturb the normal pattern of vasculogenesis. Results from this work will likely contribute to a greater understanding of vessel formation as it occurs not only in embryonic development but also in wound healing, and angiogenesis-related diseases such as ocular neovascularization, tumor growth, and hemangiomas.