CONTROL OF ANIMAL MORPHOGENESIS

他工作的长期目标是将细胞和分子 提供对动物控制的理解的方法 形态发生。这项建议侧重于三个关键的形态发生过程 所有动物胚胎的共同之处：1)胚胎细胞的定向迁移， 2)上皮片内细胞重排；3)大小调节。 这些问题将在光学清澈、可操纵的海洋中得到解决 海胆胚胎。4-D荧光显微镜将用于分析细胞 活体内的运动。将使用cDNA文库筛选来鉴定 参与这些运动的潜在调控分子。实验 是基于Ettensohn博士最近的生化研究提出的 展示特定细胞外基质作用的实验室 原肠发育过程中上皮细胞重排的决定因素。这个 尺寸调节问题将从骨骼的角度进行分析 形态发生，使用胚胎操作，细胞类型特定分子 标记物和体外骨骼生成系统。

The long-term goal of his work is to integrate cellular and molecular methods to provide an understanding of the control of animal morphogenesis. This proposal focuses on three key morphogenetic processes common to all animal embryos: 1) directional migration of embryonic cells, 2) cell rearrangements within epithelial sheets, and 3) size regulation. These problems will be addressed in the optically clear, manipulable sea urchin embryo. 4-D fluorescence microscopy is to be used to analyze cell movements in vivo. cDNA library screening will be used to identify potential regulatory molecules involved in these movements. Experiments are proposed that build on recent biochemical studies in Dr. Ettensohn's laboratory demonstrating a role for a specific extracellular matrix determinant in epithelial cell rearrangements during gastrulation. The problem of size regulation will be analyzed with respect to skeletal morphogenesis, using embryo manipulation, cell-type specific molecular markers, and an in vitro skeletogenesis system.