STUDY OF THE INHIBITORY RESPONSE OF GABA DURING CARDIOREGULATION IN LIMULUS

这个项目的长期目标是阐明心脏抑制。 在进行心脏调节的时候，在多头鳗中。γ-氨基丁酸 (GABA)和5-羟色胺(5-HT)都被认为是 灯盏花中枢神经内的心脏抑制递质 系统(CNS)。γ-氨基丁酸在人体内的精确生理作用 心脏抑制尚未得到解决。在我们澄清神经控制的努力中 在中枢神经系统中的抑制，我们假设GABA 5-羟色胺作为神经递质与5-羟色胺作为神经递质共存于中枢神经。 GABA有助于心脏调节过程中的抑制反应 中枢和外周神经系统。我们建议将GABA能 用免疫细胞化学方法观察河豚中枢神经系统内的神经元。这个 免疫反应阳性的胞体和纤维的鉴定 体节。印防己毒素和荷包牡丹碱的拮抗作用 电生理学评估，对GABA抑制反应 分析突发频率、突发内容(尖峰/突发)和突发 持续时间。我们建议进一步确定GABA能的药理学特征 在使用激动剂和拮抗剂的情况下，在中枢神经系统内使用受体。 GABA摄取和释放的动力学和药理学研究 是河豚体内假定的抑制性神经递质，在 中枢和外周神经系统的心脏调节。

The long range objectives of this project is to clarify cardioinhibition during cardioregulation in Limulus polyphemus. Gamma-aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) both have been implicated as cardioinhibitory transmitters within Limulus polyphemus Central Nervous System (CNS). The precise physiological role played by GABA in cardioinhibition is not settled. In our effort to clarify neural control of inhibition in the CNS of Limulus polyphemus, we hypothesize that GABA co-exists with 5-HT in the CNS of Limulus as a neurotransmitter, and that GABA aids in a inhibitory response during cardioregulation in both the central and peripheral nervous system. We propose to identify GABAergic neurons within the CNS of Limulus by using immunocytochemistry. The identification of immunoreactive somata and fibers to immunoreactive somata. The antagonistic action of picrotoxin, and bicuculline will be assessed electrophysiologically, on GABA inhibitory response during analysis of burst frequency, burst content (spikes/burst), and burst duration. We propose to further characterize pharmacologically GABAergic receptors within Limulus CNS with the use of agonists and antagonists. Kinetics and pharmacological studies on the uptake and release of GABA is putative inhibitory neurotransmitter in Limulus and function in cardioregulation both in the central and peripheral nervous system.