GENOTOXIC ASSAYS FOR EVALUATION OF DENTAL MATERIALS

牙科材料放置在与活组织直接接触的位置。 必须确定它们的生物效应和它们的配伍 必须对口腔组织和一般健康进行评估，以便 确保它们在患者治疗中的安全使用。 用于确定牙科材料安全性的早期协议 已将重点放在它们的一般毒性和局部组织刺激性 没有提出广泛的系统分析的财产 牙科材料的致突变性评价。因为突变可以是 与各种严重疾病有关的评估 牙科材料的诱变潜力是至关重要的。目前 关于牙齿的诱变作用的信息有限。 材料，以前大多数研究的结论是 只根据艾姆斯测试的结果。致突变性分析 目前可用的每一个都有自己的长处和弱点。 因此，一种材料或其成分的诱变潜力 不能通过单一方法可靠地确定，但必须 通过一系列测试进行了分析。 在这项拟议的调查中将检验的假设 体外Ames试验可能不能充分评估 牙科材料的遗传毒性潜力以及额外的 需要进行体内遗传毒性检测才能可靠地评估 这些材料的诱变潜力。这项研究旨在 一种复合型遗传毒性试验的应用研究 一种复合材料应用的评价程序 评价牙齿致突变性的遗传毒性试验方法 材料。以下四种遗传毒性检测方法将用于 本研究：1)体外Ames沙门氏菌微体检测；2) 体内小鼠骨髓微核试验；3)体内小鼠 精子形态检测；4)活体姐妹染色单体互换 中国仓鼠骨髓细胞的姐妹染色单体交换研究。这些测试 包括从简单的细菌到哺乳动物的各种系统 细胞，并应允许更多的压缩评估和突变 潜在的牙科材料。这项调查将确定 这些已建立的遗传毒性的适用性和适应性 用于后续评估牙科材料的测试。

Dental materials are placed in direct contact with living tissues. Their biological effects must be determined and their compatibility with oral tissues and general health must be assesed in order to insure their safe use in the treatment of patients. Earlier protocols for determining the safety of dental materials have focused on their general toxicity and local, tissue irriting properties with no extensive systematic assays proposed for mutagenic evaluation of dental materials. Because mutations can be related to a variety of serious diseases, assessment of the mutagenic potential of dental materials is essential. At present there is limited information about the mutagenic effects of dental materials, and the conclusions from most previous studies have been based only on results from the Ames Test. Mutagenicity assays currently available each have their strengths and weaknessess. Therefore, the mutagenic potential of a material or its componets cannot be reliably determined by a single method but must be analyzed by a battery of tests. The hypothesis which will be tested in this proposed investigation is that the in vitro Ames Test may not adequately evaluate the genotoxic potential of dental materials and that additional, in vivo genotoxic assays are needed in order to reliably evaluate mutagenic potential of these materials. The study is designed to investigate the application of a composite of genotoxic test procedures for evaluating the application of a composite of genotoxic test procedures for evaluating the mutagenicity of dental materials. The following four genotoxic assays will be used in this study: 1) in vitro Ames Salmonella microsome assays; 2) the in vivo mouse bone marrow micronucleus test; 3) the in vivo mouse sperm morphology test; and 4) in vivo sister chromatid exchange (SCE) studies in chinese hamster bone marrow cells. These test include a variety of systems from the simple bacterium to mammalian cells and should allow more compresensive evaluation and mutagenic potential od dental materials. This investigation will determine the applicability and adapatability of these established genotoxic tests for subsequent evaluations of dental materials.