Animal Resource and Pathology Core

核心2是提供最佳兽医护理和用于进行活体实验的设施所必需的 实验。该计划项目的S将可以访问比较医学部 AAALAC认证、集中管理和运营的面积为175,000平方米。英国《金融时报》空间，位于五栋建筑中， 研究所。在过去的八年里，每一个单元都已经建成或进行了大规模的翻新。其中四个 建筑物有三个走廊系统，其中三个有专门的生物遏制装置，以防止传染病或 危险的化学实验。用于存放无特定病原体转基因小鼠的屏障设施 蜂群也是可用的。这些动物设施为麻省理工学院的研究人员提供了一个受控的环境 进行生物医学相关研究所需的空间、设备和密封设施 动物包括无菌工作，也确保了实验过程中人员的安全。这个 DCM比较病理学实验室还将提供进行组织学检查的人员和专业知识 对体内研究提供的动物组织进行免疫细胞化学分析 计划项目的框架。具体地说，核心2的作用是提供设施、设备和 具有集中管理和监督畜牧业和实验动物专业知识的人员 操作，并提供组织病理学和转基因服务，参与在 计划项目拨款。具体目标如下： 具体目标1：提供组织学、免疫组织化学、病理学和分子表征 广泛的表型鉴定和小鼠病理损伤评估的专业知识 本计划中使用的型号 具体目标#2通过胚胎移植重新获得转基因和其他特殊小鼠品系，以确保 本计划中使用的宝石的无特定病原体状态 具体目标#3提供由项目PI和AS使用的鼠标模型的群体管理 需要通过原核显微注射DNA构建体来产生转基因动物， 胚胎干细胞操作，或使用RNAi技术的基因沉默。

Core 2 is essential for the delivery of optimal veterinary care and facilities utilized for the conduct of in vivo experimentation. The Pl.'s of this program project will have access to the Division of Comparative Medicine's AAALAC accredited, centrally managed and operated 175,000 sq. ft. of space, located in five buildings at the Institute. Each of the units has been built or extensively renovated in the last eight years. Four of these buildings have three corridor systems and three have specialized bio-containment units for infectious or hazardous chemical experimentation. Barrier facilities for housing specific pathogen free transgenic mouse colonies are also available. These animal facilities provide MIT investigators a controlled environment with the necessary space, equipment, and containment facilities for performing biomedical related research in animals including germ-free work and also ensures personnel safety during the experimental process. The DCM Comparative Pathology Laboratory will also provide the personnel and expertise to perform histological and immunocytochemical analysis of animal tissues delivered from in vivo studies performed within the framework of the program project. Specifically the role of Core 2 is to provide facilities, equipment, and personnel with expertise to centrally manage and supervise animal husbandry and experimental animal manipulations and provide histopathologic and transgenic services involved in studies conducted in the Program Project Grant. The specific aims are as follows: Specific Aim #1 To provide histology, immunohistochemistry, pathology and molecular characterization expertise for extensive phenotyping and assessment of pathological damage of mouse models used in this program Specific Aim #2 To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of GEM used in this program Specific Aim #3 Provide colony management of mouse models for use by program project Pi's and as needed generate transgenic animals by pronuclear microinjection of DMAconstructs, embryonic stem cell manipulation, or gene silencing using RNAi technology.