Imaging and Antibodies

核心B的目标是为以下人员提供成像服务、抗体和抗体生产服务： 计划项目的各个组成部分。在其他私家侦探的要求下，酒井博士将讨论 实验，设计适当的实验策略和协议，然后执行商定的 服务 成像服务：透射电子显微镜（包括免疫定位以及标准 超微结构成像）和共聚焦显微镜可用于所有项目。 抗体：根据要求，将向项目分发以下充分表征的抗体： 特异性针对β-内酰胺酶-1、β-内酰胺酶-2、LTBP-1和LTBP-4的兔多克隆抗体;单克隆抗体 对原纤蛋白和BMP-7有特异性。这些抗体都适用于小鼠材料。在 此外，核心可以提供对flbrillin-1、flbrillin-2和flbrillin-3具有特异性的单克隆抗体，以及对flbrillin-1、flbrillin-2和flbrillin-3具有特异性的单克隆抗体。 弹性蛋白，其与人、牛和禽类的原纤维蛋白反应。核心还制作并描述了 特异于人纤蛋白-4的单克隆抗体。 定制抗体：可以生成兔多克隆或小鼠单克隆抗体， 与Project Pis合作。Pis项目将提供免疫原，并将提供一些帮助 进行抗体筛选和鉴定。因为商业来源的pSmad 2/3抗体具有 不可靠和不一致，我们将合成磷酸-Smad肽用作免疫原， 产生并表征我们自己的可靠的pSmad 2/3抗体。 重组蛋白：重组蛋白的供应也有限。这些包括 重组LTBP-1、LTBP-2、LTBP-3和LTBP-4 多肽;和重组BMP-7。

The objectives of Core B are to provide Imaging services, antibodies, and antibody production services to the individual components of the Program Project. At the request of the other Pis, Dr. Sakai will discuss experiments, devise appropriate experimental strategies and protocols, and then perform agreed upon services. Imaging Services: Transmission electron microscopy (including immunolocalization as well as standard ultrastructural imaging) and confocal microscopy are available to all of the Projects. Antibodies: The following well-characterized antibodies will be distributed to the Projects upon request: rabbit polyclonal antibodies specific for fibrillin-1, fibrillin-2, LTBP-1, and LTBP-4; monoclonal antibodies specific for fibrillin and for BMP-7. These antibodies are all suitable for use with mouse materials. In addition, the Core can provide monoclonal antibodies specific for flbrillin-1, fibrillin-2, and fibrillin-3, and for elastin, which react with human, bovine, and avian fibrillins. The Core has also produced and characterized monoclonal antibodies specific for human fibulin-4. Custom-made antibodies: Rabbit polyclonal or mouse monoclonal antibodies can be generated, in consultation with Project Pis. Project Pis will provide the immunogen and will also perform some assistance with antibody screening and characterization. Because commercial sources of pSmad 2/3 antibodies have been unreliable and inconsistent, we will synthesize phospho-Smad peptides to use as immunogens, generate and characterize our own reliable pSmad2/3 antibodies. Recombinant proteins: Limited supplies of recombinant proteins are also available. These include recombinant fibrillin-1 and fibrillin-2 polypeptides; recombinant LTBP-1, LTBP-2, LTBP-3 and LTBP-4 polypeptides; and recombinant BMP-7.