Genomic Profiling Resource

许多研究人员对利用最先进的技术感兴趣，例如基于微阵列的 转录学和基因组分析，试图剖析分子图谱。在过去的十年里，我们 已经见证了研究的重大进步，这些研究直接与 基因组测序的努力。这些成果为研究者的研究提供了希望。 像癌症这样的复杂疾病。引起这些疾病的基因变化可能导致一种或几种疾病 基因组图谱异常的基因，甚至是功能紊乱的成百上千个基因。 这可能导致难以分类的基因组图谱的混杂，从而对 试图阐明癌症的前因后果的研究人员。 为了协助BCMCC调查人员解决这些问题，基因组图谱资源将整合现有的两个 贝勒医学院(BCM)设施，提供最先进的转录或基因组访问 使用微阵列技术进行分析。该资源利用斑点玻璃微阵列和商业 提供Affymetrix基因芯片平台，并在实验设计、数据分析和 生物信息学，以及对计算硬件和软件的访问。资源提供的服务 包括样本质量控制、利用微阵列进行表达谱分析、定量(实时)PCR、获取 克隆文库、斑点阵列比较基因组杂交、SNP基因分型 Affymetrix GeneChips、数据分析、培训和研讨会。由丽莎·怀特博士和刘静博士领导的 CORE将为癌症中心的研究人员提供具有成本效益的资源。

Many researchers are interested in utilizing state-of-the-art technologies such as microarray-based transcriptomic and genomic analyses to attempt to dissect molecular profiles. Within the past decade we have witnessed significant advancements in research that are directly associated with the output of the genome sequencing endeavor. The results of these achievements provide hope to investigators researching complex diseases like cancer. Alterations in genes causing these diseases may result in one or several genes having aberrant genomic profiles or even hundreds to thousands of genes with perturbed function. This can result in a mishmash of genomic profiles that is difficult to sort through, presenting a challenge to researchers attempting to elucidate the cause and effect of cancer. To assist BCMCC investigators with these issues, the Genomic Profiling Resource will integrate two existing Baylor College of Medicine (BCM) facilities to provide state-of-the-art access to transcriptional or genomic profiling using microarray technology. The Resource utilizes spotted glass microarrays and the commercially available Affymetrix GeneChip¿ platform and offers expert advice in experimental design, data analysis, and bioinformatics, as well as access to computing hardware and software. Services offered by the Resource include sample quality control, expression profiling with microarrays, quantitative (real time) PCR, access to a cDNA clone respository, comparative genomic hybridization on spotted arrays, SNP genotyping utilizing Affymetrix GeneChips, data analysis, training, and workshops. Led by Drs. Lisa White and Ching Lau, this Core will provide a cost-effective resource for Cancer Center researchers.