Integration of Core Facilities for Molecular and Cellular Characterization

描述（由申请人提供）：俄勒冈健康与科学大学（OHSU）正在申请资金，用于翻新5,431总平方英尺的核心设施空间，将整合两个重要的研究领域：流式细胞术和基因组分析。这项倡议的目标是改善分子和细胞表征的设施。Specific Aim 1巩固了流式细胞术中心的空间，该中心目前分散在OHSU校园内。具体目标2巩固了OHSU的微阵列技术空间，并为新的大规模并行测序共享资源创造了新的空间，与微阵列共享资源一起，将构成OHSU新的基因组学中心。然而，重要的是，将基因组学中心与流式细胞术中心相邻，将通过促进基因表达谱的生成和纯细胞群的DNA变异测量，创造科学协同效应。这些设施的邻近将提高向调查人员提供的服务质量，并提高这些资源整合其协同活动的效率。这些资源的整合将使方法和技术的共同开发能够最大限度地减少阵列或测序检测所需的细胞数量，并且能够将流式细胞术共享资源中的纯化细胞快速移交给基因组学中心，这将最大限度地减少基因表达谱变化的机会，并增强从少量细胞中分离完整/高质量RNA的潜力。总之，该提案的目标将通过在提供服务方面创造可持续的效率和加强科学协同作用的能力，大大扩大卫生系统资助的OHSU研究人员的资源。此外，这项建议预计将在整修阶段创造24个工作岗位，并将创造雇用长期科学和技术人员的能力。

DESCRIPTION (provided by applicant): Oregon Health & Science University (OHSU) is requesting funds to renovate 5,431 gross square feet of core facilities space that will integrate two important research areas: flow cytometry and genomic analysis. The goal of this initiative is to improve facilities for molecular and cellular characterization. Specific Aim 1 consolidates space for the Flow Cytometry Center, which is currently scattered throughout the OHSU campus. Specific Aim 2 consolidates space for OHSU's microarray technologies and creates new space for a new Massively Parallel Sequencing Shared Resource that, with the Microarray Shared Resource, will constitute the a new Genomics Center at OHSU. Importantly, however, locating the Genomics Center adjacent to the Flow Cytometry Center will create scientific synergy by facilitating the generation of gene expression profiles and DNA variation measurements from pure cell populations. The proximity of these facilities will increase both the quality of service provided to investigators and the efficiencies by which these resources can integrate their synergistic activities. Integration of these resources will enable co- development of methods and technologies to minimize the number of cells required for array or sequencing assays, and the ability to rapidly hand off purified cells from the Flow Cytometry Shared Resource to the Genomics Center will minimize the chances of changes in gene expression profiles and enhance the potential to isolate intact/high quality RNA from smaller numbers of cells. Together, the aims of this proposal will greatly expand the resources for PHS-funded researchers at OHSU by creating sustainable efficiencies in the delivery of services and enhancing the capacity for scientific synergies. In addition, this proposal is expected to create 24 jobs during the renovation phase and will create the capacity to hire long-term scientific and technical staff.