Core Rederivation and Barrier Renovation

描述（由申请人提供）：肯塔基大学（英国）要求6,432,134美元用于翻新5,526平方米。在桑德斯-布朗老年中心的四楼。拟议的改造将彻底拆除和拆除目前老化和过时的小型屏障动物设施。新设施将提供三种迫切需要的特定核心能力，这些能力目前在英国无法获得。建设并配备集中的核心胚胎冷冻保存和胚胎移植实验室。该实验室将提供内部库存和进口转基因菌株的胚胎移植再分化，以消除可能改变研究结果的致病或共生生物。此外，该实验室将为英国研究人员提供精子和胚胎冷冻保存服务，允许长期储存独特的转基因菌株，作为持续繁殖的替代方案，或作为意外群体损失事件的研究资源。该实验室将毗邻一个小型的高安全性无菌屏障，用于在转移到传统动物设施之前进行再衍生菌株的初始扩展。最后，将建造一个用于非生物啮齿动物住房的房间，并配备三个柔性薄膜隔离器，以支持对具有明确微生物菌群的转基因菌株的迅速增长的需求。通过将这三个设施定位在同一个地点，我们将最大限度地利用现有的支持灭菌设备来提供一套核心服务，这些服务已被调查人员确定为关键的基础设施需求。

DESCRIPTION (provided by applicant): The University of Kentucky (UK) requests $6,432,134 to renovate 5,526 sq. ft. on the fourth floor of the Sanders-Brown Center for Aging. The proposed renovation will completely demolish and remove the present aged and obsolete small barrier animal facility. The new facility will provide three critically needed specific core capabilities that are presently not available at UK. A centralized core embryo cryopreservation and embryo transfer laboratory will be constructed and outfitted. This laboratory will provide embryo transfer rederivation of both in-house stock and imported transgenic strains to eliminate pathogenic or commensal organisms potentially altering research results. Additionally this laboratory will provide both sperm and embryo cryopreservation services to UK investigators permitting the long- term storage of unique transgenic strains as an alternative to continual breeding or as a research resource in the event of unanticipated colony loss. This laboratory will be adjacent to a small high- security sterile barrier for initial expansion of rederived strains prior to movement to conventional animal facilities. Finally, a room for gnotobiotic rodent housing will be constructed and outfitted with three flexible film isolators to support the burgeoning need for transgenic strains with defined microbial flora. By locating these three facilities in this single location we will maximally leverage the available support sterilization equipment to provide a set of core services that have been identified by the investigators as a critical infrastructure need.