Vitreous Proteome and Inflammatory Mediators in Ocular Inflammatory Disease

这项研究将检测患有葡萄膜炎或眼部炎症的人的蛋白质。玻璃体是一种无色透明物质，填充在晶状体后面的眼球中，随着新微技术的使用，评估玻璃体现在成为可能。现在有机会评估严重的、非传染性的、危及视力的葡萄膜炎中存在的蛋白质种类。 在美国国立卫生研究院参加多中心葡萄膜炎类固醇治疗(MASH)研究的18岁及以上患者可能有资格参加这项研究。最终可能会有多达200名患者入选。研究人员将研究在植入类固醇植入物的手术中将从患者眼睛中取出的玻璃体。类固醇植入物被用来代替免疫抑制疗法，这是一种减少免疫系统活动的方法。 患者将接受一项手术，在眼睛上打一个小孔，植入植入物。通常情况下，在这一过程中会有少量的玻璃体出来，在这项研究中，玻璃体标本将被用于检测炎症产物。与此同时，将采集一小部分血液样本，约1-1/2汤匙，以便研究人员将血液中可能存在的炎症产物与玻璃体中的炎症产物进行比较。如果患者在研究期间需要再次放置植入物，他或她将被要求允许采集玻璃体和血液样本，就像以前一样。收集的样本不会被用来诊断患者的病情或改变正在进行的任何治疗。所有样本都将被贴上特殊的代码编号，这样就没有关于患者的识别信息。这项研究不会涉及患者的检查或预定的探视。

This study will examine the proteins of people with uveitis, or inflammation of the eyes. Evaluating the vitreous, the colorless transparent substance that fills the eyeball in back of the lens, is now possible with the use of new microtechnology. There is an opportunity to evaluate the kinds of proteins that are present in severe, noninfectious sight-threatening uveitis. Patients ages 18 and older who have been enrolled in the Multicenter Uveitis Steroid Treatment (MUST) study at NIH may be eligible for this study. Up to 200 patients eventually may be enrolled. Researchers will study the vitreous that will be removed from patients' eyes during an operation to insert a steroid implant. The steroid implant is used instead of immunosuppressive therapy, a way to reduce the action of the immune system. Patients will undergo a procedure involving a small hole made in the eye into which the implant is placed. Normally a small amount of the vitreous comes out during that procedure, and in this study, the vitreous specimen will be taken for testing of inflammatory products. At the same time, a small sample of blood, about 1-1/2 tablespoons, will be collected so that the researchers can compare inflammatory products that may be in the blood with those in the vitreous. If a patient needs to have the implant placed again during the study, he or she would be asked permission for collection of the vitreous and blood samples, as previously. Samples collected will not be used to diagnose patients' conditions or to change any treatments being done. All samples will be labeled with special code numbers so that there is no identifying information about patients. This study will not involve examinations or scheduled visits of patients.