Image-Guided Hydrodynamic Gene Delivery

(在此处输入文本，这是您的应用程序的新摘要信息。此部分不得超过30行文本。) 本申请是为响应美国国立卫生研究院宣布可为竞争性修订申请(非OD-09-058)提供恢复法案资金而编写的。本申请中概述的实验旨在扩大父基金(RO1EB007357)的特定目标、研究设计和方法的范围，该基金的标题为“图像引导的流体动力基因传递”。 父母资助的目标是开发一种图像引导的流体动力基因传递系统，用于将特定部位的基因传递到肝脏。自去年项目启动以来，我们已经建立了以猪为动物模型的程序，并证明了图像引导的流体动力基因传递对于肝脏基因传递是高效和安全的。在这项竞争性修订申请中，我们建议通过将非人类灵长类动物作为进一步发展的额外动物模型来扩大研究范围。我们计划系统地评估图像引导的流体动力学基因在狒狒体内传递的有效性，以便建立可用于开发用于人类基因传递的计算机程序的流体动力学参数。其具体目的是：(1)研究不同注射压力和不同体积的DNA溶液对恒河猴肝脏基因传递效率的影响；(2)评价转基因表达的持续性和流体力学方法对转基因动物的长期影响。 为了配合科学研究步伐的加快和/或为了创造和保留就业机会，我们将为拟议的研究聘请两名全职员工。此外，动物的使用也将为那些动物护理专业人员创造就业机会。拟议中使用的试剂也将有助于创造或留住在研究试剂公司工作的人。

(Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text.) This application was written in response to the NIH Announcement for the Availability of Recovery Act Funds for Competitive Revision Applications (NOT-OD-09-058). The experiments outlined in this application were designed to expand the scope of the specific aims, research design, and methods of the parent grant (RO1 EB007357) entitled “Image-guided Hydrodynamic Gene Delivery”. The objective of the parent grant is to develop an image-guided hydrodynamic gene delivery system for site-specific gene delivery to the liver. Since the start of the project last year, we have established the procedure using pigs as an animal model and demonstrated that the image-guided hydrodynamic gene delivery is highly effective and safe for liver gene delivery. In this competitive revision application we propose to expand the study by including the non-human primate as an additional animal model for further development. We plan to systematically assess the effectiveness of the image-guided hydrodynamic gene delivery in baboons in order to establish the hydrodynamic parameters that can be used for development of a computer program for gene delivery in humans. The specific aims are: (1) To study the effect of selected injection pressures and volumes of DNA solution on gene delivery efficiency to baboon liver, and (2) to assess the persistency of transgene expression and the long-term effect of hydrodynamic procedure on the transfected animals. In compliance to Acceleration of the Tempo of Scientific Research and/or Allow for Job Creation and Retention, We are going to hire two full time employees for the proposed study. In addition, the use of animals will also create job for those who are animal caring professionals. The reagents used in the proposed would also help in creating or retaining people who work in research reagent companies.