Zeiss LSM 710 confocal microscope

描述(由申请人提供)：本提案请求支持购买蔡司LSM 710共焦显微镜，这将是纽约大学医学院五个主要用户实验室工作的关键共享资源，也是更广泛的纽约大学医学院社区外部用户的宝贵资源。主要用户实验室的项目主要由6个NIH R01赠款资助，研究在胚胎发育期间如何指定细胞类型，以及这些不同类型的细胞如何重新排列以形成功能器官。这些研究包括：(1)确定斑马鱼胚胎心脏分化和形态发生的机制(Yelon)；(2)研究果蝇眼睛的图案和全局转录控制在细胞命运决定中的作用(Treisman)；(3)确定线虫早期胚胎细胞和上皮细胞的极化方式(Nance)；(4)了解线虫胚系干细胞如何分化和增殖(Hubbard)；以及(5)破译斑马鱼维管系统如何形成图案(Torres-Vazquez)。每个项目在很大程度上依赖于荧光显微镜的使用来显示细胞的形状和运动，并检查蛋白质的亚细胞定位；因为成像的胚胎或组织相对较厚，需要共聚焦显微镜来去除平面外的荧光。能够使用可光激活的蛋白质并同时对GFP和mCherry进行成像的辅助激光被要求能够实现新型和强大的实时成像实验。主要用户PI是高级和初级教员的组合，具有相当多的共焦显微镜经验。他们将与图像核心设备负责人Alice Leung博士合作，支持和操作共焦显微镜。梁博士还将监督整个纽约大学医学院外部调查人员对该仪器的培训和使用。公共卫生相关性：这项提案中要求的显微镜将对推进纽约大学医学院正在进行的NIH资助项目的研究至关重要，所要求的辅助激光线路将使新的调查路线成为可能。总体而言，这些项目使用模型生物来解决发育生物学中的基本问题，包括细胞是如何形成模式和组织成器官的。对这些过程的基本了解对于理解人类发展和疾病至关重要。

DESCRIPTION (provided by applicant): This proposal requests support for the purchase of a Zeiss LSM 710 confocal microscope, which will be a shared resource crucial to the work of five Major User laboratories at NYU School of Medicine as well as a valuable resource to outside users in the wider NYU School of Medicine community. The projects of the Major User labs, which are funded predominantly by 6 NIH R01 grants, address both how cell types are specified during embryonic development and how these different types of cells are rearranged to form functional organs. These studies include (1) identifying mechanisms of heart differentiation and morphogenesis in zebrafish embryos (Yelon); (2) investigating patterning of the Drosophila eye and the role of global transcriptional control in cell fate decisions (Treisman); (3) determining how C. elegans early embryonic cells and epithelial cells polarize (Nance); (4) learning how germ-line stem cells in C. elegans differentiate and proliferate (Hubbard); and (5) deciphering how the zebrafish vasculature becomes patterned (Torres-Vazquez). Each project relies heavily on the use of fluorescence microscopy to visualize cell shapes and movements and to examine the subcellular localization of proteins; because the embryos or tissues imaged are relatively thick, confocal microscopy is required to remove out-of-plane fluorescence. Accessory lasers that enable use of photoactivatable proteins and simultaneous imaging of GFP and mCherry are requested to enable new and powerful types of live imaging experiments. The Major User PIs are a combination of senior and junior faculty with considerable confocal microscopy experience. Together with Dr. Alice Liang, head of the Image Core Facility, they will cooperate in supporting and operating the confocal microscope. Dr. Liang will also oversee training and use of the scope by outside investigators throughout NYU School of Medicine. PUBLIC HEALTH RELEVANCE: The microscope requested in this proposal will be crucial in advancing the research of NIH-funded projects ongoing at NYU School of Medicine, and the requested accessory laser lines will enable new lines of investigation. Collectively the projects use model organisms to address fundamental questions in developmental biology, including how cells are patterned and organized to form organs. A basic understanding of these processes is critical to understanding human development and disease.