Zeiss Laser Scan Microscope (LSM) 510-duo

描述（由申请人提供）：共聚焦显微镜技术的最新进展为理解细胞生物学及其与其他细胞的相互作用开辟了新的途径。例如，现在我们可以检查活细胞，我们可以研究蛋白质对刺激的实时反应易位。使用多光子激光器，可以到达更深的深度，因此整个胚胎、胰岛、整个脂肪垫或其他小结构可以用光学切片彻底检查，而不是物理切片。随着新的软件，现在这些共聚焦显微镜，复杂的分析，无论是钙通量，解混光谱重叠，FRET或FRAP的便利。这些细胞生物学方法是提高对正常代谢生理学以及导致1型和2型糖尿病及其相关并发症的病理生理过程的理解的关键。本申请申请蔡司激光扫描显微镜（LSM） 510 Duo的资金，这将被添加到美国国立卫生研究院资助的糖尿病和内分泌研究中心在乔斯林糖尿病中心。该仪器将由乔斯林的高级显微镜设施进行操作和维护。该仪器的使用率估计为75-100%，分布在至少18名nih资助的Joslin研究人员和2名外部同事之间。研究项目涉及1型和2型糖尿病及其并发症。这些研究涉及糖尿病研究的许多领域，包括与1型糖尿病相关的自身免疫、干细胞、胰岛功能和再生、胰岛素抵抗机制、2型糖尿病、肥胖、代谢和血管并发症。公共卫生相关性：糖尿病已达到流行病的流行程度，不仅对美国，而且对全世界的卫生保健费用造成越来越大的负担。乔斯林糖尿病中心位于朗伍德医疗区，与哈佛医学院密切相关，其独特的使命是提供有关糖尿病的医疗保健、研究和教育，以改善糖尿病患者的生活。这种最先进的共聚焦显微镜，加上本应用程序中列出的研究人员的专业知识和研究，将使人们对细胞代谢和糖尿病的原因和后果有新的认识，并为糖尿病的治疗带来新的进展

DESCRIPTION (provided by applicant): Recent advances in technology of confocal microscopy have opened new avenues for understanding the biology of cells and their interactions with other cells. For example, now that we can examine living cells, we can study the translocation of proteins in response to stimulation in real time. With the multiphoton lasers, greater depth can be reached so that whole embryos, islets, whole fat pads or other small structures can be examined thoroughly with optical, rather than physical, sectioning. With the new software that is now available with these confocal microscopes, sophisticated analyses, whether of calcium flux, unmixing spectral overlap, FRET or FRAP are facilitated. These cell biological approaches are key to an improved understanding of normal metabolic physiology as well as the pathophysiological processes leading to types 1 and 2 diabetes and associated complications. This application requests funds for a Zeiss Laser Scanning Microscope (LSM) 510 Duo, which will be added to the NIH-funded Diabetes and Endocrinology Research Center at the Joslin Diabetes Center. This instrument will be operated and maintained by the Advanced Microscopy Facility at Joslin. The usage of this instrument is estimated at 75-100%, distributed between at least 18 NIH-funded investigators at Joslin and 2 of our external colleagues. The research projects are relevant to both type 1 and type 2 diabetes and associated complications. These studies address a number of areas of diabetes research including autoimmunity related to type 1 diabetes, stem cells, islet function and regeneration, mechanisms of insulin resistance, type 2 diabetes, obesity, metabolism and vascular complications. PUBLIC HEALTH RELEVANCE: Diabetes has reached epidemic prevalence causing an increasing burden to the health care costs not just of the United States but also the world. The Joslin Diabetes Center, located within the Longwood Medical Area and closely tied to Harvard Medical School, is uniquely focused with a mission to provide health care, research and education on diabetes that will improve the lives of people with diabetes. This state-of-the-art confocal microscope together with the expertise and research of investigators listed in this application should lead to new understanding into cellular metabolism and of the causes and consequences of diabetes and to new advances for the treatment of diabetes