Acquisition of a Liquid Chromatography-Triple Quadrupole/Linear Ion Trap Mass Spe

描述(由申请人提供)：本申请请求提供资金，用于购买一台混合三重四极杆/线性离子陷阱质谱仪(4000Q陷阱；应用生物系统)，该质谱仪连接到一个液色谱(LC)系统。新仪器将安置在彭宁顿生物医学研究中心(PBRC)的蛋白质组学核心设施中。该仪器是支持美国国立卫生研究院资助的十多名研究肥胖、糖尿病和衰老研究人员的项目的关键组成部分。这些项目涉及脂类、脂肪酸、代谢物、多肽和蛋白质翻译后修饰的检测、鉴定、表征和量化。该仪器将允许这些研究人员使用质谱学(MS)技术进行生物分子分析，这是目前PBRC或任何邻近机构都无法获得的。该机构与路易斯安那州立大学(LSU)农业中心、新奥尔良的LSU-健康科学中心(HSC)、什里夫波特的LSU-HSC、杜兰国家灵长类研究中心以及南路易斯安那州地区的小型私营公司的其他研究人员合作。这些调查人员还将获得该仪器提供的技术。目前，利用LC-MS/MS方法对蛋白质、多肽、脂质、碳水化合物和代谢物进行分析，通常用于鉴定具有生物医学意义的分子。这些生物分子在血浆或组织样本等复杂基质中的定量方法需要进一步的验证、确认和表征。三重四极杆的高特异性和线性离子陷阱的高灵敏度相结合，使4000Q陷阱成为这些项目的理想选择。在所有质量分析仪中，使用选择性和多反应监测(SRM和MRM)扫描功能实现的定量的选择性和限度是最好的。4000Q陷阱还具有MS3分析能力，允许在一次注射中识别和详细描述生物分子的结构。使用信息依赖采集，这些扫描模式可用于各种组合，包括一些不太常规的组合，例如MRM/增强乘积离子和MRM/MS3，取决于应用和对灵敏度、选择性和/或量化的需要。这种扫描模式的组合是任何其他仪器都无法实现的。仪器将由训练有素的人员操作和维护。蛋白质组学核心设施主任将安排日常运作，并向用户提供技术专长。一个咨询委员会将确保接触到主要用户和其他NIH支持的调查人员，并将负责该仪器的长期管理。公共卫生相关性：NIH资助的研究人员将使用4000 Q陷阱质谱仪来探索导致肥胖、糖尿病和衰老的机制，并了解控制生物钟和血脑屏障的生物途径。该仪器提供的独特分析工具还将用于开发生物标记物和设计针对每种疾病状态的治疗方法。

DESCRIPTION (provided by applicant): This application requests funding for the acquisition of a hybrid triple quadrupole/linear ion trap mass spectrometer (4000 Q TRAP; Applied Biosystems) interfaced to a liquid chromatography (LC) system. The new instrument will be housed in the Proteomics Core Facility at the Pennington Biomedical Research Center (PBRC). This instrument is a critical component to support NIH-funded projects of more than ten investigators studying obesity, diabetes and aging. These projects involve detection, identification, characterization and quantification of lipids, fatty acids, metabolites, peptides and protein post-translational modifications. This instrument will allow these investigators to use mass spectrometric (MS) techniques for biomolecular analysis that are currently not available at PBRC or at any neighboring institutions. The facility collaborates with other researchers at Louisiana State University (LSU) Agricultural Center, LSU-Health Sciences Center (HSC) in New Orleans, LSU-HSC in Shreveport, Tulane National Primate Research Center, and small private companies in the South Louisiana region. These investigators will also have access to the technologies offered by this instrument. Today, the analysis of proteins, peptides, lipids, carbohydrates and metabolites using LC-MS/MS methods is commonly performed to identify molecules of biomedical significance. Methods for quantification of these biomolecules in complex matrices such as blood plasma or tissue samples are required for further verification, validation and characterization. The combination of high specificity of the triple quadrupole and high sensitivity of linear ion trap makes 4000 Q TRAP ideal for these projects. The selectivity and limit of quantification achieved using selected and multiple reaction monitoring (SRM and MRM) scan functions are best among all mass analyzers. The 4000 Q TRAP also has MS3 analysis capability which allows for identification and detailed structural characterization of biomolecules in a single injection. Using information dependent acquisition, these scan modes can be used in a variety of combinations including some less conventional combinations such as MRM/Enhanced product ion and MRM/MS3 depending on the application and need for sensitivity, selectivity and/or quantification. This combination of scan modes cannot be achieved by any other instrument. The instrument will be operated and maintained by trained personnel. The Director of the Proteomics Core Facility will schedule daily operation and provide technical expertise to the users. An advisory committee will ensure access to the major users and other NIH-supported investigators and will be responsible for the long term management of the instrument. PUBLIC HEALTH RELEVANCE: The 4000 Q TRAP mass spectrometer will be utilized by the NIH-funded investigators to explore mechanisms leading to obesity, diabetes, and aging and to understand biological pathways controlling circadian clock and blood brain barrier. The unique analytical tools offered by this instrument will also be used to develop biomarkers and to devise therapy for each disease state.