BD FACSAria II

描述（由申请人提供）：本申请资金用于购买最先进的流式细胞仪，即BD Biosciences FACSAria II系统，以支持华盛顿大学细胞分析设施（CAF）的现有共享资源设施。CAF自1989年成立以来由免疫学系建立和运营，支持大量教师及其实验室，其NIH资助的研究依赖于他们使用流式细胞仪。在过去的一年中，来自六所不同学校或学院和区域灵长类动物中心的23个不同部门的82名主要研究人员的实验室使用了该设施，代表了非常多样化的研究兴趣和系统。该申请记录了增加分选机访问和访问FACAria II的高级功能的需求，这将使华盛顿大学的用户在他们的方法中更具创造性，并在他们能够识别和纯化感兴趣的细胞的参数中更加严格。公共卫生相关性：流式细胞仪细胞分选是一项关键技术，生物医学科学家可以通过这项技术从人类和人类健康和疾病的相关动物模型中分离和详细研究细胞，特别是包括与血液学、免疫学、传染病和恶性疾病以及再生和基因疗法的发展有关的研究。此应用程序要求一个新的BD生物科学FACSAria II流式细胞仪，这将提高在这些和相关领域的研究范围在华盛顿大学提供最好的可用的流式细胞仪技术的动手访问。

DESCRIPTION (provided by applicant): This application requests funds to purchase a state-of-the art flow cytometric cell sorter, a BD Biosciences FACSAria II system in support of existing shared resource facilities at the Cell Analysis Facility (CAF) of the University of Washington. The CAF, established and operated by the Department of Immunology since its inception in 1989, supports a large number of faculty and their laboratories, whose NIH funded research is dependent upon their access to flow cytometric instrumentation. During the past year, laboratories of 82 principal investigators from 23 different departments in six different Schools or Colleges and the Regional Primate Center have used the facility, representing an extraordinarily diverse range of research interests and systems. This application documents the need for increased sorter access and for access to the FACAria II's advanced features, which will allow users at the University of Washington to be more creative in their approaches and rigorous in the parameters by which they are able to identify and purify cells of interest. Public Health Relevance: Flow cytometric cell sorting is a key technology through which biomedical scientists can isolate and study in detail cells from humans and relevant animal models of human health and disease, notably including studies relevant to hematological, immunological, infectious and malignant diseases and to the development of regenerative and gene therapies. This application requests a new BD Biosciences FACSAria II flow cytometric cell sorter, which will enhance the scope of research in these and related areas at the University of Washington by providing hands-on access to the best available flow cytometric technology.