Spinning Disk Confocal Microscope System with Photobleaching, Photoactivation, an

描述（申请人提供）：需要一台3I旋转盘共聚焦显微镜，用于对活细胞进行灵敏的荧光成像。该显微镜将服务于范德比尔特大学医学院和艺术与科学学院多个部门的8个主要用户和一些次要用户。用户需要在仪器中对活细胞的动态过程进行共聚焦荧光成像，以提供最大的灵敏度和空间分辨率，最小的光漂白和光毒性，并且易于使用。他们还希望对活标本进行光漂白、光活化和光消融。我们的8个主要用户都是在共聚焦显微镜经验丰富，并提供文件显示，新的CSU22旋转Nipkow磁盘技术提供了活细胞成像的改进，远远超出了范德比尔特大学其他传统共聚焦显微镜所提供的。3I系统提供的改进包括：在最小的漂白或损伤的情况下连续监测活细胞；检测常规仪器无法看到的微弱和微小细节；CFP/GFP/YFP/RFP成像激光选项；用于微弱信号动态跟踪的增强相机包，用于同时FRAP或光激活的马赛克单元，以及用于脉冲染料细胞激光漂白，烧蚀和切割的微点系统。3I显微镜将安装在集中的显微镜室，在那里进行监督和维护。该申请得到了广泛的兴趣、需求和大学的财政支持，并将服务于美国国立卫生研究院资助的大型科学界。公共卫生相关性：旋转圆盘共聚焦显微镜将使我们能够成像活细胞中的各种过程。我们特别感兴趣的是使用该仪器来更好地了解细胞迁移，这是许多生物过程的基础，包括胚胎发生，伤口愈合，炎症反应，以及病理过程，如癌症，动脉粥样硬化，关节炎和先天性发育性脑缺陷。更好地了解这些过程可以为这些疾病带来新的治疗方法。

DESCRIPTION (provided by applicant): A 3I spinning disk confocal microscope is requested for sensitive fluorescence imaging of living cells. The microscope will serve 8 major users and a number of minor users from multiple departments throughout Vanderbilt University Medical School and College of Arts & Sciences. The users require confocal fluorescence imaging of dynamic processes in living cells in an instrument that provides maximum sensitivity and spatial resolution, minimal photobleaching and phototoxicity, and ease of use. They also wish to perform photobleaching, photactivation and photoablation of live specimens. Our 8 major users are all experienced in confocal microscopy and provide documentation showing that the new CSU22 spinning Nipkow disk technology provides improvements for live cell imaging that are vastly beyond those afforded by other conventional confocal microscopes at Vanderbilt University. The improvements provided by the 3I system include: continuous monitoring of live cells in time-lapse with minimal bleaching or damage; detection of faint and minute details not visible in conventional instruments; laser options for CFP/GFP/YFP/RFP imaging; intensifier-camera package for dynamic tracking of faint signals, Mosaic unit for simultaneous FRAP or photoactivation, and Micropoint system for pulsed dye cell laser bleaching, ablation and cutting. The 3I microscope will be installed in a centralized microscope room where it will be supervised and maintained. The application is supported by a broad base of interest, need, and financial support by the university and will serve a large NIH-funded scientific community. PUBLIC HEALTH RELEVANCE: A spinning disk confocal microscope will allow us to image various processes in living cells. We are particularly interested in using this instrument to gain a better understanding of cell migration, which is fundamental to many biological processes, including embryogenesis, wound healing, and the inflammatory response, as well as pathological processes, such as cancer, atherosclerosis, arthritis and congenital developmental brain defects. A better understanding of these processes could lead to new therapeutic approaches for these disorders.