Clinical Transplant Centers

临床移植中心核心将由10个主要的学术移植中心组成 美国每年总共进行约1360例成人肾移植。它的活动 将通过临床数据库、该计划与三个科学项目相结合 项目网站、年度科学会议和10个临床组之间的积极中期互动 调查员和首席调查员。我们对这一核心的重点目标是提供 具有必要的患者材料和相关临床数据的项目，以验证以下假设： 1)PBL和肾移植活检组织中的基因表达和蛋白质组特征 确定与活检证实的急性排斥反应和慢性移植物肾病有关，2) 基因和蛋白质表达谱将提供对参与的分子途径的洞察力 宿主免疫反应和供体器官对移植的反应，3)充分性 免疫抑制的定义是缺乏排斥相关基因和蛋白的表达 可在任何给定时间由基因和蛋白质表达谱确定，以及4)靶向SNP 基因分型和候选基因序列研究将确定基因之间的联系 供者和受者与移植事件和结果以及基因相关 和蛋白质表达特征。围绕所有这些假设，我们建议研究 种族(高加索人、非裔美国人和西班牙裔)和/或性别在基因上 至少影响移植后表达的基因和蛋白质的一个子集，并与临床相关 结果。一个关键点是正确的患者亚组和临床名称 成功完成注册和样本获取，这是该核心的两项主要任务， 将决定整个计划项目中所有科学家的成功潜力。

The Clinical Transplant Center Core will be comprised of 10 major academic transplant centers in the United States doing approximately 1360 adult kidney transplants per year in total. Its activities will be integrated with the three Scientific Projects through the Clinical Database, the Program Project website, yearly scientific meetings and active interim interactions between the 10 Clinical Investigators and the Principal Investigators. Our focused objectives for this Core are to provide the Projects with the requisite patient materials and correlating clinical data to test the hypotheses that: 1) gene expression and proteomic signatures in PBL and kidney transplant biopsies can be identified that correlate with biopsy-proven acute rejection and chronic allograft nephropathy, 2) gene and protein expression profiles will provide insights into the molecular pathways involved in both the host immune response and the donor organ's response to transplantation, 3) the adequacy of immunosuppression as defined by the absence of rejection-related gene and protein expression can be determined at any given time by gene and protein expression profiles, and 4) targeted SNP genotyping and candidate gene sequence studies will identify connections between the genetics of the donor and the recipients that correlate with transplant events and outcomes as well as with gene and protein expression signatures. Arching over all these hypotheses, we propose to examine the possibility that race (Caucasian, African American and Hispanic) and/or sex will genetically influence at least a subset of genes and proteins expressed post transplant and correlate with clinical outcomes. A critical point is that the correct clinical designations of the patient subgroups and successful completion of enrollment and specimen acquisitions, the two primary tasks for this Core, will determine the potential of success for all the scientists in the entire Program Project.