Proteomics and Genomics Core

蛋白质组学和基因组学核心

• 批准号: 8324850
• 负责人: Daniel R. Salomon
• 金额: $ 34.04万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8324850
• 关键词: Allografting; Bioinformatics; Biological; Biological Markers; Biotechnology; Blood Cells; California; Cell Separation; Cells; Clinical Trials; Collaborations; Complex; Core Facility; DNA; DNA Microarray Chip; DNA Sequencing Facility; Doctor of Philosophy; Event; Exons; Faculty; Failure; Funding; Gene Expression; Genes; Genome; Genomics; Grant; Head; Human; Immune response; Informatics; Infusion procedures; Institutes; Instruction; Islets of Langerhans Transplantation; Kidney Transplantation; Laboratories; Leadership; Letters; Mass Spectrum Analysis; Medicine; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Messenger RNA; Metric; Modeling; Molecular; Nucleic Acids; Outcome; Principal Investigator; Proteins; Proteomics; Protocols documentation; RNA; RNA Sequences; Recording of previous events; Regulation; Reperfusion Injury; Research; Research Institute; Research Personnel; Resources; Sampling; Services; Specialist; Staging; Structure; Supervision; Technology; Time; Tissues; Translational Research; Translations; Transplantation; United States National Institutes of Health; Universities; Work; base; design; experience; functional genomics; mass spectrometer; next generation; nonhuman primate; programs; regenerative; response; square foot; success; tandem mass spectrometry

PROJECT SUMMARY (See instructions): The Genomics and Proteomics Core will be composed of six functional sub-cores located within The Scripps Research Institute. Each sub-core is organized with a clear leadership structure and they are designed to be functionally complementary to the Program's objectives: Core 1 - Sample Receiving Center Core 2 - Gene Expression Microarrays Core 3 - Next Generation Sequencing Core 4 - Quantitative PCR Core 5 - Mass Spectrometry Proteomics Core 6 - Genomic Informatics The primary objectives of the Genomics and Protemics Core will be to provide the investigators in Projects 1 and 2 with cutting edge technologies and supportive expertise for functional genomics including DNA microarrays, tandem mass spectrometry proteomics and next generation deep RNA sequencing. These technologies will be applied to parallel studies of carefully designed nonhuman primate models of islet and kidney transplantation. Experimentally-defined transplant outcomes will be the focus including the activation of mesenchymal stem cells (MSC), graft infiltrating cells during different stages of rejection or successful tolerance, and peripheral blood cells and constituent subsets of biological significance such as Tregs as a function of time after transplantation and MSC infusions and with success or failure. Finally, the Core will develop a new nonhuman primate DNA microarray with Affymetrix based on the latest exon probe technology that will advance the present work and provide the entire field with a new resource. The ultimate objective is to support the translation of a successful and optimized protocol for MSC-based transplantation therapy into a human clinical trial, creating genomic metrics for high quality MSC isolations and immunological biomarkers for the impact of MSC on the transplant immune response

项目总结（见说明）： 基因组学和蛋白质组学核心将由位于斯克里普斯研究所内的六个功能子核心组成。每个次级核心都有明确的领导结构，其目的是在功能上与方案的目标相辅相成： 核心1 -样品接收中心 核心2 -基因表达微阵列 核心3 -下一代测序 核心4 -定量PCR 核心5 -质谱蛋白质组学 核心6 -基因组信息学 基因组学和蛋白质组学核心的主要目标是为项目1和2的研究人员提供功能基因组学的尖端技术和支持性专业知识，包括DNA微阵列，串联质谱蛋白质组学和下一代深度RNA测序。这些技术将应用于精心设计的非人灵长类动物胰岛和肾移植模型的平行研究。实验定义的移植结果将是重点，包括间充质干细胞（MSC）的活化，移植物浸润细胞在排斥或成功耐受的不同阶段，外周血细胞和生物学意义的组成子集，如Tblast作为移植后时间和MSC输注后的函数，成功或失败。最后，核心将开发一个新的非人灵长类动物DNA微阵列与Affyphin基于最新的外显子探针技术，这将推进目前的工作，并提供整个领域的新资源。最终目标是支持将基于MSC的移植治疗的成功和优化方案转化为人类临床试验，创建高质量MSC分离的基因组指标和MSC对移植免疫应答影响的免疫学生物标志物