A sensitive multiplexed diagnostic platform using disposable 2D paper networks

描述（由申请人提供）：本申请涉及广泛的挑战领域（06）使能技术和特定的挑战主题，06-EB-102，生物医学技术和系统的开发。需要快速、易于使用且成本非常低的多重诊断测定用于护理点（POC），特别是在低资源环境中。微流控系统可以将实验室质量的多重检测应用于POC应用，但到目前为止，它们需要台式“阅读器”，在大多数低资源环境中使用成本太高。侧流测定成本低，易于由未经训练的人使用，但通常每个装置仅测量一种分析物，并且不能进行用于复杂扩增和样品预处理步骤的多步化学处理，导致较低的测试灵敏度和再现性。我们建议将微流控回路的复杂性与常规侧向流测定的简单性联合收割机相结合。我们计划展示一种新的诊断平台，二维纸网络（2DPN），可以在没有仪器的情况下执行复杂的化学过程，并且每次测试的成本与传统的侧向流测试相当。具体而言，我们建议使用一次性2DPN在低资源环境中使用，以证明一种灵敏的多重免疫测定。该平台将具备以下能力：（1）从2滴血检测多个靶，（2）得到结果的时间小于10分钟，（3）机载样品制备，（4）试剂干燥储存，（5）可由未经训练的人使用，（6）与常规侧流测定相比提高的灵敏度，以及（7）与信用卡相当的装置尺寸，重量小于100克。

DESCRIPTION (provided by applicant): This application addresses broad Challenge Area (06) Enabling Technologies and specific Challenge Topic, 06-EB-102, Development of Biomedical Technologies and Systems. Multiplexed diagnostic assays that are rapid, easy to use, and very low cost are needed for use at the point of care (POC), especially in low-resource settings. Microfluidic systems can bring laboratory-quality multiplexed assays to POC applications, but so far they require benchtop "readers" that cost too much for use in most low-resource settings. Lateral flow assays are low cost and easy to use by untrained people, but generally measure only one analyte per device, and are not capable of multi-step chemical processing for sophisticated amplification and sample pretreatment steps, resulting in lower test sensitivity and reproducibility. We propose to combine the sophistication of the microfluidic circuit with the simplicity of the conventional lateral flow assay. We plan to show that a novel diagnostic platform, two-dimensional paper networks (2DPNs), can be used without an instrument to perform complex sets of chemical processes and at a cost-per-test that will be comparable to conventional lateral flow tests. Specifically, we propose to demonstrate a sensitive multiplexed immunoassay using disposable 2DPNs for use in low-resources settings. The platform will have the following capabilities; (1) detection of multiple targets from 2 drops of blood, (2) a time to result of less than 10 minutes, (3) on-board sample preparation, (4) reagents stored dry, (5) usable by an untrained person, (6) improved sensitivity compared to conventional lateral flow assays, and (7) a device size comparable to a credit card with weight less than 100 gm.