A sensitive multiplexed diagnostic platform using disposable 2D paper networks

描述（由申请人提供）：本申请涉及广泛的挑战领域（06）使能技术和特定的挑战主题，06- eb -102，生物医学技术和系统的发展。在护理点（POC），特别是在资源匮乏的环境中，需要快速、易于使用且成本极低的多路诊断测定。微流体系统可以为POC应用带来实验室质量的多路分析，但到目前为止，它们需要台式“读取器”，在大多数低资源环境中使用成本太高。横向流动分析成本低，易于未经培训的人员使用，但通常每个设备只能测量一种分析物，并且无法进行复杂的扩增和样品预处理步骤的多步骤化学处理，导致测试灵敏度和重复性较低。我们建议将微流控电路的复杂性与传统侧流分析的简单性结合起来。我们计划展示一种新的诊断平台，二维纸网络（2DPNs），可以在没有仪器的情况下使用，以执行复杂的化学过程集，每次测试的成本将与传统的横向流动测试相当。具体地说，我们建议在低资源环境下使用一次性2DPNs进行敏感的多路免疫分析。该平台将具有以下功能：(1)从2滴血液中检测多个目标，(2)一次不到10分钟的结果，(3)机载样品制备，(4)试剂干燥储存，(5)未经训练的人员可用，(6)与传统的横向流动测定相比提高了灵敏度，(7)设备大小与信用卡相当，重量小于100克。

DESCRIPTION (provided by applicant): This application addresses broad Challenge Area (06) Enabling Technologies and specific Challenge Topic, 06-EB-102, Development of Biomedical Technologies and Systems. Multiplexed diagnostic assays that are rapid, easy to use, and very low cost are needed for use at the point of care (POC), especially in low-resource settings. Microfluidic systems can bring laboratory-quality multiplexed assays to POC applications, but so far they require benchtop "readers" that cost too much for use in most low-resource settings. Lateral flow assays are low cost and easy to use by untrained people, but generally measure only one analyte per device, and are not capable of multi-step chemical processing for sophisticated amplification and sample pretreatment steps, resulting in lower test sensitivity and reproducibility. We propose to combine the sophistication of the microfluidic circuit with the simplicity of the conventional lateral flow assay. We plan to show that a novel diagnostic platform, two-dimensional paper networks (2DPNs), can be used without an instrument to perform complex sets of chemical processes and at a cost-per-test that will be comparable to conventional lateral flow tests. Specifically, we propose to demonstrate a sensitive multiplexed immunoassay using disposable 2DPNs for use in low-resources settings. The platform will have the following capabilities; (1) detection of multiple targets from 2 drops of blood, (2) a time to result of less than 10 minutes, (3) on-board sample preparation, (4) reagents stored dry, (5) usable by an untrained person, (6) improved sensitivity compared to conventional lateral flow assays, and (7) a device size comparable to a credit card with weight less than 100 gm.