Animal Model Core

项目总结(见说明)： 动物模型核心B将提供项目1、2和3中使用的所有老鼠。核心将负责 订购小鼠，进行诱导实验性结肠炎所需的各种操作，维持 特定的无病原体屏障设施，执行常规检查，以确保在 并协助从小鼠身上采集组织。将这些功能集中在一个动物模型核心中 为不同项目中正在研究的MICE提供统一性和质量控制，降低成本，并将 通过确保它们在项目中的最佳使用，帮助节省所需的MICE数量。的第二个目标 动物模型的核心是由特伦顿·舍布博士进行的集中病理分析， 一个专业的兽医病理学家。来自不同项目的组织切片在解释之前被编码 并在需要的地方进行量化评分。这促进了项目之间的互动，也有利于 他们之间的结果比较。动物模型核心的第三个目的是从基因上提供 改良的转基因和基因敲除小鼠品系，用于各种项目的实验。所有的 上述目标已在上一个周期中实现。关于第三个目标，在以前的 在项目1、2和3中已经产生了许多新的转基因小鼠品系， 在解决针对微生物区系的先天和适应性免疫方面非常有价值，这是 本计划项目的中心目标。这些小鼠系包括一个CBirl鞭毛蛋白T细胞受体转基因小鼠 小鼠、IL-10/Thy1.1报告小鼠(10BiT)、IL-17F/Thy1.1报告小鼠和IFNy/Thyl.l 记者老鼠。这些鼠标线将被安置在核心B中，并可用于 这份续签申请书。查理斯·埃尔森博士将担任主任，凯西·韦弗博士将担任联合 这个核心里的导演。比较医学系的萨姆·卡特纳博士将担任 顾问。一个由每个项目的PI加上Schoeb和Cartner博士组成的监督委员会将 监督核心的使用和功能。

PROJECT SUMMARY (See instructions): Animal Model Core B will provide all mice used in Projects 1, 2, and 3. The Core will be responsible for ordering mice, performing various manipulations required to induce experimental colitis, maintaining a specific pathogen-free barrier facility, performing roufine surveys, to ensure absence of pathogens in the colony, and assist in harvesting tissues from mice. Centralization of these functions in an Animal Model Core provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects. A second aim of the Animal Model Core is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects are coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results between them. A third purpose of the Animal Model Core is to provide genetically modified transgenic and knockout mouse strains for use in experiments in the various projects. All of the above Aims have been accomplished in the previous cycle. In regard to the third Aim, during the previous cycle a number of novel genetically-modified mouse lines have been generated in Projects 1, 2, and 3 that have been extremely valuable in addressing innate and adaptive immunity to the microbiota, which is the central goal of this Program Project. These mouse lines include a CBirl flagellin T cell receptor transgenic mouse, an IL-10/Thy1.1 reporter mouse (lOBiT), an IL-17F/Thy1.1 reporter mouse, and an IFNy/Thyl.l reporter mouse. These mouse lines will be housed in Core B and made available to the different projects in this renewal application. Dr. Charies Elson will serve as Director and Dr. Casey Weaver will serve as Co- Director in this Core. Dr. Sam Cartner from the Department of Comparative Medicine will serve as Consultant. An Oversight Committee consisting of the PI of each Project plus Drs. Schoeb and Cartner will oversee the usage and function of the Core.