NEW CHEMICAL TOOLS FOR THE QUANTITATIVE ASSESSMENT OF BIOLOGICAL METABOLOMES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 代谢酶通过激活或抑制基因表达和酶活性在调节生物功能中起关键作用。因此，系统中代谢物的定量允许生物样品的比较分析（系统生物学）。开发能够全面定量测量代谢物的技术将是恢复代谢平衡和减轻某些疾病症状的关键第一步。 为了定量和比较代谢物，必须用同位素标记物选择性地标记它们，使得分子易于通过光谱技术识别。本文提出了新的化学试剂，其将靶向最普遍的代谢物官能团，并允许对生物系统的大量关键代谢物进行定量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metabolites play a key role in regulating biological function by activating or inhibiting both gene expression and enzyme activity. Therefore, the quantification of metabolites in a system allows for the comparative analysis of biological samples (systems biology). The development of technologies that allow for the comprehensive quantitative measurement of metabolites will be a crucial first step in restoring metabolic balance and mitigating symptoms of certain diseases. In order to quantify and compare metabolites, they must be selectively labeled with isotopic markers such that molecules are readily recognized by spectroscopy techniques. Novel chemical reagents are herein proposed that will target the most prevalent metabolite functional groups and allow for the quantification of a large number of the key metabolites of biological systems.