Molecular and Genomic Imaging Center

描述：（由申请人提供）我们在此提出分子和基因组成像中心（MGIC），以响应生物医学界对灵活、经济、高分辨率技术的需求，在基因组和转录组水平上识别和表征生物系统的变异。我们计划通过开发聚合酶菌落（polony）技术来帮助满足这一需求。Polonies代表了一种高度平行的核酸分析方法，它是现实的、近距离的、模块化的和通用的。MGIC的主要任务是有效地将来自技术开发人员的各种贡献集成到一个强大的平台中，该平台可以顺利地传播给具有专业临床和生物学兴趣的各种用户。这些是主要目的：(1)高度平行荧光原位测序。(2)转录组的单分子分析，特别是神经分化和哺乳动物选择性剪接。(3)直接分子单倍型和远程序列连通性。(4)单细胞DNA和RNA的表征，特别是哺乳动物干细胞中不对称细胞分裂的表征。(5)处理核酸分析中的组合模式和空间模式的计算算法和系统建模。(6) ELSI组件侧重于将技术转化为临床应用的问题以及对匿名概念的挑战。

DESCRIPTION: (provided by applicant) We propose here the Molecular and Genomic Imaging Center (MGIC) in response to a biomedical-community-wide need for flexible, cost-effective, high-resolution technology to identify and characterize variation in biological systems at the level of genomes and transcriptomes. We plan to help meet this need by developing the polymerase colony, or polony, technology. Polonies represent a highly parallel method of nucleic acid analysis that is realistic, close-at-hand, modular, and versatile. The primary mission of the MGIC is to efficiently integrate a diverse set of contributions from technology developers into a robust platform that can be smoothly disseminated to a variety of users with specialized clinical and biological interests. These are the main aims: (1) Highly parallel fluorescent in situ sequencing. (2) Single molecule profiling of the transcriptome, in particular of neural differentiation and of mammalian alternative splicing. (3) Direct molecular haplotyping and long-range sequence connectivity. (4) Characterization of DNA & RNA from single cells, in particular characterizing asymmetric cell division in mammalian stem cells. (5) Computational algorithms and systems modeling addressing combinatorial and spatial patterns in nucleic acid analysis. (6) An ELSI component focuses on issues of translation of technology to clinical applications and challenges to the concepts of anonymity.