Experimental Mice

9.1.3实验小鼠共享服务 实验小鼠共享服务（EMSS）是一个一站式的共享服务，为您的所有在体小鼠 实验需要。EMSS的目的是为亚利桑那州癌症中心的研究人员提供 （AZCC）采用实验小鼠模型和广泛的小鼠实验技术。的 EMSS结合了以前的基因工程小鼠"发育"共享服务（GEMSS） 和之前的EMSS因此，EMSS从基因打靶的设计和构建开始， 转基因小鼠载体，基因打靶和ES细胞筛选靶向克隆，生产基因打靶的 小鼠，转基因小鼠的生产，转基因和敲除创始人的筛选，胚胎 重新衍生，用于病原体清除和小鼠品系的冷冻保存，直至完成实验 在异种移植和GEM模型的各个方面提供服务。EMSS的具体目标包括： 模型，维护共享和研究者集落，协助设计体内实验，以及 由具有小鼠实验方案专业知识的人员进行小鼠实验。所以 EMSS提供小鼠实验内和实验之间程序的一致性和质量， 实验结果的准确性，结果解释的可信度，以及 小鼠实验，这对于实验室内和实验室间的比较分析至关重要。原因是 小鼠是NIH类型资助以及临床进展的所有转化癌症工作的模型。 试验，EMSS是一项绝对必要的共享服务。EMSS的好处是提供AZCC 研究人员具有成本效益的机制，用于创建不同的小鼠模型，并完成 利用训练有素和经验丰富的技术人员进行体内小鼠实验。EMSS继续扩大 以非常具有成本效益和效率的方式提供服务。

9.1.3 EXPERIMENTAL MOUSE SHARED SERVICE The Experimental Mouse Shared Service (EMSS) is a one-stop shared service for all your in vivo mouse experimentation needs. The purpose of the EMSS is to provide investigators at the Arizona Cancer Center (AZCC) with experimental mouse models and with a wide range of mouse experimentation techniques. The EMSS has combined the previous Genetically Engineered Mouse "Developmental" Shared Service (GEMSS) and the previous EMSS. Therefore, the EMSS begins with the design and construction of gene targeting and transgenic mouse vectors, gene targeting and ES cell screening for targeted Clones, production of genetargeted mice, production of transgenic mice, screening for transgenic and knockout founders, embryo rederivation for pathogen cleanup and cryopreservation of mouse strains through to complete experimentation services in all aspects of xenograft and GEM models. The specific objectives of the EMSS include creation of models, maintenance of shared and investigator colonies, assistance in the design of in vivo experiments, and performance of mouse experiments by personnel with expertise in mouse experimental protocols. Hence, the EMSS provides consistency and quality of procedures within and between mouse experiments that ensures accuracy in experimental results, confidence in interpretation of results, and an inner-consistency between mouse experiments which is essential for intra-lab and inter-lab comparative analyses. Due to the fact that the mouse is a model for all translational cancer work for both NIH-type funding as well as progression to clinical trials, the EMSS is an absolutely essential shared service. The benefits of the EMSS are to provide AZCC investigators with a cost-effective mechanism for the creation of different mouse models and for completing in vivo mouse experiments utilizing highly trained and experienced technicians. The EMSS continues to expand its services in a very cost-effective and efficient manner.