MAINTENANCE AND REPAIR OF THE IVEM F20

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在本报告所述期间(2007年4月1日至2007年4月30日)，拍摄了相当于921个倾斜系列的照片(1x2蒙太奇被算作2个倾斜系列)。平均每周使用时间约为31.6小时(56周总计1770小时)。由于F30显微镜今年的可操作性更强，F20的使用量比去年有所下降。在这台显微镜上对5名新使用者进行了塑料和冷冻断层扫描的培训，取得了很大的成功(93.75小时)。我们有140.75个小时的服务和开发。从2006年9月1日开始，我们开始通过打卡和下班来记录工作时间，而不是仅仅记录总小时数。根据这些数据，我们已经确定，大约22%的显微镜时间是在下班后(下午5点到上午8点，北京时间和周末)。图1说明了F20的用法。塑料切片断层扫描(包括蒙太奇)，紧随其后的是冷冻成像(单粒子和样品质量评估)，常规电磁工作(网格、阴性染色样本的预筛选、连续切片工作、单粒子工作和免疫-EM)是F20使用最多的工作，其次是低温断层扫描、服务、开发(硬件和软件)和新用户培训。图2说明了已完成的层析成像功的分布(总共进行了921次倾斜序列)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this reporting period (4/1/06-4/30/07), an equivalent of 921 tilt-series were taken (a 1X2 montage was counted as 2 tilt-series). The average use is about 31.6 hours/week (1770 total hours in 56 weeks). Because the F30 microscope was more operational this year, the F20 usage dropped from last year. Training of five new users in both plastic and cryo tomography was performed on this microscope with great success (93.75 hours). We had 140.75 hours of service and development. From September 1, 2006, we started keeping track of hours by clocking in and out vs. just total hours logged. From that data, we have determined that about 22% of microscope time is after hours (5pm-8am M-F and weekends). Figure 1 illustrates F20 usage. Plastic section tomography (including montaging), closely followed by cryo imaging (single particle and evaluation of sample quality), regular EM work (pre-screening of grids, negative stain samples, serial section work, single-particle work, and immuno-EM) comprised the largest use of the F20, followed by cryo tomography, service, development (hardware and software) and training of new users. Figure 2 illustrates the distribution of tomography work done (a total of 921 tilt series taken).