SURFACTANT PROTEIN B EXPRESSION IN AVIAN LUNG

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 萨布丽娜·麦加里(布劳赫) 表面活性蛋白B在禽肺中的表达 禽肺在结构上与哺乳动物的上呼吸道相似，被认为是一种新的人类上呼吸道疾病模型。特别令人感兴趣的是表面活性蛋白质。人肺泡表面活性蛋白(SP-A、SP-B、SP-C和SP-D)由2型细胞分泌，具有降低肺泡表面张力和调节局部免疫反应的功能。上呼吸道表面活性物质的组成和功能还不是很清楚，但它可能会阻止细支气管壁的塌陷，并介导炎症反应。禽类旁支管(作为气体交换场所的小管)含有2型细胞，并已被证明表达SP-A和SP-B。除此之外，对鸟类肺脏的研究还很少。本项目的目标是(1)鉴定表面活性蛋白在禽肺中的表达，并确定表达上调的胚龄；(2)建立禽胚肺组织的体外培养体系；(3)建立禽2型细胞的分离和分化模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sabrina McGary (Brougher) Surfactant Protein B Expression in Avian Lung The avian lung, which is structurally similar to mammalian upper airway, has been suggested as a novel model for human upper respiratory disease. Of particular interest are the surfactant proteins. The human alveolar surfactant proteins (SP-A, SP-B, SP-C and SP-D) are secreted by type 2 cells and function to reduce alveolar surface tension and modulate a localized immune response. The composition and function of surfactant in the upper airway is less well understood, yet it likely prevents the collapse of the bronchioles, and mediates inflammatory responses. The avian parabronchi (tubules which serve as the site of gas exchange) contain type 2 cells and have been shown to express SP-A and SP-B. Beyond this, little research has been conducted on avian lung. The goals of this project are (1) to characterize expression of the surfactant proteins in avian lung, and identify the embryonic age at which upregulation of expression occurs; (2) develop an in vitro culture system for avian embryonic lung explant tissue, and (3) develop a model for the isolation and differentiation of avian type 2 cells