Group Training in Product Development and Manufacturing

专性细胞内病原体的繁殖和量化既劳动密集型又昂贵 承诺。这些特点，再加上伯氏梭菌的高度传染性和选择性 指定，使最基础的研究工作复杂化。然而，因为高度提纯和 所列举的伯内蒂隐孢子虫对拟议的研究是必不可少的，该研究是BSL 3的专用核心设施 遏制，训练有素的技术人员，并提出了一个档案系统。因此，目的是 核心B是向蒙大拿州的4个Coxiella项目中的每个项目提供培养方面的专业知识， 伯氏柯克斯体1期和2期的纯化、计数和存档。核心将位于 蒙大拿州立大学，BSL-3设施的位置，将由艾伦·哈姆森 蒙大拿州立大学。 项目之间的协作。核心B将为四个研究项目中的每个项目提供服务 蒙大拿州柯克西拉小组以及任何其他需要他们服务的RMRCE调查员。这些服务将 包括制作标准的伯氏柯克斯体1期和2期疫苗，以及狂犬病病毒株 Coxiella，以及标准化Coxiella计数和Coxiella生物体档案服务。在……里面 此外，这两名兼职技术人员在BSL-3中接受了专门处理小鼠肺部感染的培训。 当不从事其他活动时，核心B技术人员将可协助4个蒙大拿州 柯克西拉预计他们会在几天内牺牲老鼠，因为这些时间点是劳动密集型的。我们的BSL3 SOP 要求“伙伴制度”，个人不允许单独在设施内工作。它远不止如此 拥有两个兼职浮动职位比每个单独的研究项目更划算 资助全职技术人员来覆盖这些偶尔的、劳动密集型的时间点。因此，核心是 对每个项目的目标来说是不可或缺的和关键的，并需要项目之间的一致性，以便 项目之间的结果可以直接进行比较。 核心B将支持RMRCE关于免疫调节、佐剂和疫苗的综合研究重点 (IRF 1)以及细菌治疗(IRF 2)，其资源将由RPS 1.1、1.2、1.3和 2.4.

Propagation and quantification of obligate intracellular pathogens are both labor-intensive and costly undertakings. These characteristics, coupled with C. burnetii's highly infectious nature and select agent designation, complicate the most basic research endeavors. However, because highly purified and enumerated C. burnetii is essential to the proposed research, a dedicated core facility with BSL 3 containment, highly trained technical personnel, and an archival system is proposed. Thus, the purpose of the Core B is to make available to each of the 4 Montana Coxiella projects expertise in the cultivation, purification, enumeration, and archiving of phase 1 and 2 Coxiella burnetii. The core will be located at Montana State University, the location of the BSL-3 facilities, and will be directed by Allen Harmsen of Montana State University. Collaborations among projects. Core B will provide services to each of the 4 research projects of the Montana Coxiella group as well as any other RMRCE investigator needing their service. These services will include making standardized inocula of phase 1 and 2 Coxiella burnetii as well as the Crazy strain of Coxiella, as well as the services of standardized Coxiella enumeration and archival of Coxiella organisms. In addition, the two part- time technicians are trained specifically in doing lung infections in mice in the BSL-3. When not engaged in their other activities, the Core B technicians will be available to assist the 4 Montana Coxiella projects on days they sacrifice mice since these time points are labor intensive. Our BSL3 SOP requires a "buddy system" and individuals are not allowed to work alone in the facility. It is much more economical to have two part time floating positions rather than for each of the individual research projects to fund full time technicians to cover these occasional, labor-intensive time points. Therefore, the core is integral and critical to the goals of each of the projects and required for consistency between projects so that results between projects can be directly compared. Core B will support the RMRCE Integrated Research Foci on Immunomodulation, Adjuvants and Vaccines (IRF 1) as well as Bacterial Therapeutics (IRF 2), and its resources will be utilized by RPs 1.1, 1.2, 1.3, and 2.4.