Imaging Hyaloid Regression

描述（由申请人提供）：在透镜形成过程中，形成一个短暂的血管网络，即玻璃体网络，在透镜发育时为其提供营养。然而，在人类胎儿发育后期和小鼠出生后早期发育期间，玻璃体血管经历退化，以使通过透镜的视觉路径透明。玻璃体脉管系统的异常退化或生长导致几种疾病，包括持续性增生性晶状体图尼卡膜（PHTVL）、持续性增生性原始玻璃体（PHPV）、持续性胎儿脉管系统（PFV）和持续性瞳孔前膜（PPM）。在这项研究中，我们将使用荧光报告小鼠标记参与血管消退的关键细胞类型，实时共聚焦成像和FACS分选和表达分析，以确定导致血管消退发作的细胞事件。 公共卫生相关性：眼睛血管的异常生长和退化是儿童、成人和老年人失明的主要原因（Campochiaro，2000）。本研究将使用活体成像来表征新生儿眼睛中的血管如何退化，使用小鼠模型，其中玻璃体血管中的单个细胞类型用荧光蛋白报告分子标记。将使用实时成像和FACS分选来定义启动血管消退的新事件。

DESCRIPTION (provided by applicant): During the formation of the lens, a transient vessel network, the hyaloid network, forms to nourish the lens as it develops. However, during late fetal development in humans and early postnatal development in mice, hyaloid vessels undergo regression to enable a transparent path of vision through the lens. Abnormal regression or growth of the hyaloid vasculature results in several diseases, including persistent hyperplastic tunica vasculosa lentis (PHTVL), persistent hyperplastic primary vitreous (PHPV), persistent fetal vasculature (PFV) and persistent prepupillary membrane (PPM). In this study, we will use fluorescent reporter mice labeling key cell types involved in vessel regression, live confocal imaging and FACS sorting and expression analysis to define cellular events leading to the onset of vessel regression. PUBLIC HEALTH RELEVANCE: The abnormal growth and regression of blood vessels in the eye is the leading cause of blindness in children, adults and the elderly (Campochiaro, 2000). This study will use vital imaging to characterize how vessels regress in the newborn eye using mouse models in which individual cell types in the hyaloid vasculature are labeled with fluorescent protein reporters. Live imaging and FACS sorting will be used to define novel events in that initiate vessel regression.