Study of O2 Activation by Mossbauer Spectroscopy

描述（由申请人提供）：拟议的研究旨在阐明含铁酶的结构和功能，这些含铁酶参与核酸、氨基酸、脂肪酸代谢、芳香族化合物生物降解和抗生素生物合成中的各种生化反应。这项研究将得到高价合成复合物的研究的补充，这些复合物可以作为生物系统的结构或电子模型。这些研究将集中在制备和表征铁的高价态，如FeIV和FeV。还建议继续研究酵母线粒体，特别是产生与各种神经变性疾病有关的三价铁聚集体的缺失突变体。所采用的实验技术是穆斯堡尔谱和电子顺磁共振。研究计划还包括基于密度泛函理论计算的理论研究。具体地，将研究以下系统：（1）高原儿茶酸2，3双加氧酶、三种Rieske双加氧酶和甲烷单加氧酶。(2)合成的单核FeIV和FeV复合物以及双核FeIVFeIII和FeIVFeIV复合物，可以作为铁蛋白活性位点的结构和/或电子模型。(3)涉及铁-四氨基大环配体的活化剂，在绿色化学中具有已证实的潜力，并已被证明在杀死炭疽孢子和消除我们供水中的污染物方面非常有效。(4)完整的线粒体来自S.酿酒厂，以确定条件下，特别是强度的穆斯堡尔谱可以用来解决重要的健康问题。公共卫生相关性参与氧活化的含铁酶影响所有生物系统中的许多化学转化。对这些酶的拟议研究，加上对模拟生物系统结构和功能特征的新型合成复合物的研究，将揭示这些重要酶的功能。这项研究将得到一系列合成复合物研究的补充，这些复合物已被证明在绿色化学中有效，在杀死炭疽孢子等病原体方面非常有效。

DESCRIPTION (provided by applicant): The proposed research seeks to elucidate the structure and function of iron-containing enzymes involved in a diverse range of biochemical reactions in the metabolism of nucleic acids, amino acids, fatty acids, biodegradation of aromatic compounds, and the biosynthesis of antibiotics. This research will be complemented by the study of high-valent synthetic complexes that can serve as structural or electronic models of the biological systems. The studies will focus on preparing and characterizing high-valent states of iron such as FeIV and FeV. It is also proposed to continue studies of yeast mitochondria, in particular of deletion mutants that produce aggregates of ferric iron which have been implicated in various neurodegenerative diseases. The experimental techniques employed are M"ssbauer spectroscopy and electron paramagnetic resonance. The research plan also includes theoretical studies based on density functional theory calculations. Specifically the following systems will be studied: (1) Homoprotochatechuate 2,3 dioxygenase, three Rieske dioxygenases, and methane monooxygenase. (2) Synthetic mononuclear FeIV and FeV complexes as well as dinuclear FeIVFeIII, and FeIVFeIV complexes that can serve as structural and/or electronic models for the active sites of iron proteins. (3) Activators involving Fe-tetraamido macrocyclic ligands that have a demonstrated potential in green chemistry and have proven very potent in killing anthrax spores and eliminating pollutants from our water supply. (4) Intact mitochondria from S. cerevisiae, to identify conditions where the particular strength of M"ssbauer spectroscopy can be employed to address important health problems. PUBLIC HEALTH RELEVANCE Iron-containing enzymes involved in oxygen activation affect numerous chemical transformations in all biological systems. The proposed studies of these enzymes, coupled with investigations of novel synthetic complexes that mimic structural and functional features of the biological systems, will reveal how these important enzymes function. The research will be complemented by studies of a family of synthetic complexes that have proven to be effective in green chemistry and extremely potent in killing pathogens such as anthrax spores.