Cellular Neuroscience Core

该IDDRC的最初（1999年）申请包括神经病理学和神经科学的独立核心，后者提供细胞培养方面的专业知识。随着用户需求的发展，这些设施显然应该合并为一个单一的细胞神经科学核心。这是在1994年完成的，快乐博士担任主任。2001年，他担任这一职务，当时领导权移交给杰弗里·戈尔登博士，他是一名儿科神经病理学家，主要研究发育障碍。Golden博士于1997年首次加入CHOP， 立即成为从事IDDRC，无论是作为一个用户，并作为细胞神经科学核心的副主任。 自成立以来，该核心已为用户提供了多种最先进的方法，用于可视化正常，发育中的神经细胞和经历各种形式的变性和再生的神经细胞中基因产物的分布。为了更好地满足用户的需求，我们不断地增加新的技术、仪器和试剂。1995年，在儿童医院的慷慨援助下，我们购买了一台徕卡共聚焦显微镜，并在其中添加了倒置显微镜， 显微操作器/显微注射器，以及安装在工作台上的环境室，从而允许 在生理条件下长时间观察和操作活细胞。以这种方式，我们提供了8色容量荧光成像。我们的机构支付了设备的费用，我们使用IDDRC的资金来部分支持技术人员。 我们对核心库进行了其他几项值得注意的技术补充，包括切片和整个胚胎的原位杂交。我们的总体目标不仅是提供一种技术，而且是一种咨询服务，以促进方法的实施和数据的解释。我们坚持这一政策时，我们还增加了视频增强显微镜，以更好地支持IDDRC研究人员在分析细胞内Ca 2+和Na+以及线粒体电位的估计。

The initial (1999) application for this IDDRC included separate cores in Neuropathology and Neuroscience, with the latter offering expertise in cell culture. As user needs evolved, it became apparent that these facilities should be merged into a single Cellular Neuroscience Core. This was accomplished in 1994 with Dr. Pleasure as the Director. He served in this capacity unfil 2001, when leadership passed to Dr. Jeffrey Golden, a pediatric neuropathologist with a primary interest in developmental disorders. Dr. Golden first joined CHOP in 1997 and immediately became engaged with the IDDRC, both as a user and as an Associate Director of the Cellular Neuroscience Core. Since its inception this Core has provided users with a diverse repertoire of state-of-the-art methods for visualizaion of the distribufions of gene products in normal, developing neural cells and in those undergoing various forms of degeneration and regenerafion. We have confinually and eagerly added new skills, instrumentafion and reagents to better serve our users' needs. In 1995, with the generous assistance of the Children's Hospital, we purchased a Leica confocal microscope to which we added inverted microscopy, stagemounted micromanipulators/microinjectors, and a stage-mounted environmental chamber, thus permitting prolonged observation and manipulation of living cells under physiological conditions. In this manner we offered 8-color capacity fluorescent imaging. Our institution paid for the apparatus and we used IDDRC funding to partially support a technician. We made several other notable technological additions to the Core repertoire, including in situ hybridization in both sections and whole embryos. Our general purpose has been not only to make available a technology, but a consultative sen/ice that facilitates implementation of the method as well as the interpretation of data. We adhered to this policy when we also added video-enhanced microscopy in order to better support IDDRC investigators in analyzing intracellular Ca2+ and Na+ as well as the estimate of mitochondrial potential.