Gene Expression Profiling On Insulin Resistance And Obesity

我们正在进行全球外显子表达谱分析，以补充正在进行的位置克隆工作，以确定糖尿病及其预测因素、胰岛素抵抗、胰岛素分泌功能障碍和肥胖的易感基因。为此，我们正在使用Affymetrix外显子阵列来检测非糖尿病全血PIMA印第安人组织样本中的外显子表达谱，这些外显子阵列查询了整个基因组中100多万个已知和预测的外显子。 我们收集了225个骨骼肌组织样本和250个脂肪组织样本 来自非糖尿病皮马印第安人，他们已经使用高胰岛素正血糖钳在体内对身体成分和胰岛素作用进行了代谢表型分析。这些志愿者中的大多数在7-10年后的糖尿病状态的随访数据是可用的，因此将能够识别该人群中的糖尿病前外显子表达谱。我们已经完成了所有骨骼肌和脂肪组织样本的外显子阵列，目前正在分析这些数据，并使用单核苷酸多态性对我们的全基因组关联研究中收集的许多受试者的基因数据进行比较。

We are performing global exon expression profiling to complement the ongoing positional cloning effort to identify susceptibility genes for diabetes and its predictors, insulin resistance, insulin secertory dysfunction and obesity. To this end, we are examining exon expression profiles in tissue samples from non-diabetic full-blooded Pima Indians using Affymetrix exon arrys that query over 1 million known and predicted exons across the entire genome. We have collected 225 skeletal muscle tissue samples and 250 adipose tissue samples from non-diabetic Pima Indians who have been metabolically phenotyped for body composition and insulin action in vivo using the hyperinsulinemic euglycemic clamp. Follow up data is available on the diabtic status of most of these volunteers, up to 7-10 years later, so will be able to identify a prediabtic exon expression profile in this population. We have completed the exon arrays on all skeletal muscle and adipose tissue samples and are currently analyzing these data and comparing to genotypic data collected on many of the subjects from our genome-wide association study using single nucleotide polymorphisms.