Gene Expression Profiling On Insulin Resistance And Obesity

我们正在进行全球外显子表达谱分析，以补充正在进行的定位克隆工作，以确定糖尿病及其预测因子，胰岛素抵抗，胰岛素分泌功能障碍和肥胖的易感基因。为此，我们正在检查外显子表达谱的组织样本，从非糖尿病的全血皮马印第安人使用Affytek外显子arrys查询超过100万个已知的和预测的外显子在整个基因组。 我们收集了225个骨骼肌组织样本和300个脂肪组织样本 来自非糖尿病的皮马印第安人，他们已经使用高胰岛素正葡萄糖钳夹对体内身体组成和胰岛素作用进行了代谢表型分析。大多数这些志愿者的糖尿病状态的随访数据是可用的，长达7-10年后，因此将能够确定该人群中的糖尿病前外显子表达谱。在过去的一年里，我们已经完成了所有骨骼肌样本的外显子阵列，并从脂肪样本中提取了RNA，这些样本已经准备好进行分析。

We are performing global exon expression profiling to complement the ongoing positional cloning effort to identify susceptibility genes for diabetes and its predictors, insulin resistance, insulin secertory dysfunction and obesity. To this end, we are examining exon expression profiles in tissue samples from non-diabetic full-blooded Pima Indians using Affymetrix exon arrys that query over 1 million known and predicted exons across the entire genome. We have collected 225 skeletal muscle tissue samples and 300 adipose tissue samples from non-diabetic Pima Indians who have been metabolically phenotyped for body composition and insulin action in vivo using the hyperinsulinemic euglycemic clamp. Follow up data is available on the diabtic status of most of these volunteers, up to 7-10 years later, so will be able to identify a prediabtic exon expression profile in this population. In the past year we have completed the exon arrays on all skeletal muscle samples and have extracted the RNA from the adipose samples and these are ready for analysis.