A Mass Spectrometry System for Quantitative Proteomics

描述（申请人提供）：这是一份申请资金购买Q质谱仪和液相色谱系统用于定量蛋白质组学研究的申请。该仪器将被整合到罗伯特伍德约翰逊医学院/罗格斯大学生物质谱设备中。目前设施中的仪器已达到最大容量，我们需要额外的能力为13名nih资助的研究人员进行定量蛋白质组学实验。我们的用户群体将应用定量质谱法最有效地解决广泛的重要生物医学研究领域，包括神经系统疾病、细菌和病毒感染、癌症、过敏反应、糖尿病、消化系统疾病和睡眠障碍。具体的实验包括：在整个昼夜周期中，时钟蛋白的水平变化和翻译后修饰的量化，了解溶酶体及其在人类神经退行性疾病中的作用的系统生物学方法，模拟胃消化条件下蛋白质食物过敏原表位的持久性，丙型肝炎病毒、逆转录病毒和衣原体在肿瘤发生过程中抑癌因子翻译后修饰的动力学和生命周期不同阶段病原体-宿主相互作用的分析。因此，所要求的仪器将是我们研究界的一项重要资产，并将加快美国国立卫生研究院资助的罗格斯大学和明尼苏达大学罗伯特·伍德·约翰逊医学院的基础和应用卫生研究的步伐。

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase a Q Exactive mass spectrometer and liquid chromatography system that will be used for quantitative proteomics research. The instrument will be integrated into the Robert Wood Johnson Medical School/Rutgers University Biological Mass Spectrometry Facility. Current instrumentation in the Facility is used at maximal capacity and we require additional capabilities to conduct quantitative proteomics experiments for thirteen NIH-funded investigators. Our user group will apply quantitative mass spectrometry to most efficiently address a wide range of important biomedical research areas including neurological disease, bacterial and viral infection, cancer, anaphylaxis, diabetes, digestive disease, and sleep disorders. Specific experiments include quantification of changes in levels and post-translational modifications of clock proteins throughout the circadian cycle, a systems biology approach to understand the lysosome and its role in human neurodegenerative disease, persistence of epitopes on protein food allergens under conditions that simulate gastric digestion, dynamics of post- translational modification of tumor suppressors during oncogenesis and analysis of pathogen-host interactions at different stages in their life cycle for hepatitis C virus, retroviruses, and chlamydia.. The requested instrument will therefore represent a significant asset to our research community and will accelerate the pace of basic and applied NIH-funded health-research at Rutgers University and the Robert Wood Johnson Medical School of UMDNJ.