Diffuse Intrinsic Pontine Glioma Genomic Study

从尸检标本中收集了18块石蜡包埋的组织块，这些组织块来自NCI和外部机构治疗的患者。目前正在接受病理确认和复查。将从该区块收集具有代表性的肿瘤区域。DNA将通过标准程序从核心中提取。肿瘤DNA将被加工成高分辨率的比较基因组杂交。将使用阵列数据的循环二进制分段来识别拷贝数变化的区域。将对样本中的共识区域进行识别和询问，以寻找与肿瘤进展相关的基因和途径。这项研究的结果在2009年神经肿瘤学会会议上公布。这项研究将扩大到包括所有级别的胶质瘤。

Eighteen paraffin-embedded tissue blocks obtained from autopsy specimens have been collected from patients treated at NCI and outside institutions. These are currently undergoing pathology confirmation and review. A representative area of tumor will be collected from the block. DNA will be extracted from the core with standard procedures. Tumor DNA will be processed for high resolution comparative genomic hybridization. Regions of copy number change will be identified using circular binary segmentation of the array data. Consensus regions across specimens will be identified and interrogated for genes and pathways related to tumor progression. Results of this study were presented at the Society of Neuro-Oncology meeting 2009. The study will be expanded to include all grades of gliomas.